DICP OpenIR
Subject Area物理化学
Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry
Zhao, Jieyu; Hu, Chunxiu; Zeng, Jun; Zhao, Yanni; Zhang, Junjie; Chang, Yuwei; Li, Lili; Zhao, Chunxia; Lu, Xin; Xu, Guowang; Xu GW(许国旺)
KeywordCapillary Electrophoresis Mass Spectrometry Plant Metabolomics Method Validation Tobacco Leaf
Source PublicationMETABOLOMICS
2014-10-01
DOI10.1007/s11306-014-0631-4
Volume10Issue:5Pages:805-815
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectEndocrinology & Metabolism
WOS Research AreaEndocrinology & Metabolism
WOS KeywordSOLID-PHASE MICROEXTRACTION ; FALSE DISCOVERY RATE ; LIQUID-CHROMATOGRAPHY ; GAS-CHROMATOGRAPHY ; CE-MS ; METABOLOMICS ; EXTRACTION ; PLANT ; LEAVES ; OPTIMIZATION
AbstractMany metabolites in plant are highly polar and ionic. Their analysis using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry can be problematic. Therefore a capillary electrophoresis-mass spectrometry (CE-MS) method with charge-driven separation characteristic was developed to investigate polar metabolites in tobacco. To obtain as many features as possible, extraction of polar metabolites was optimized by the design of experiments and evaluated by univariate statistics. Method validation was carried out to evaluate the analytical characteristics including calibration curve, precision, sample stability and extraction reproducibility. The developed method was successfully applied in studying 30 tobacco leaves obtained from Yunnan and Guizhou provinces in China. A total of 154 polar metabolites were identified based on available database. Multivariate pattern recognition clearly revealed the metabolic differences between the two geographic areas and 43 significantly different metabolites were defined by the non-parametric hypothesis test (Mann-Whitney U test) and false discovery rate. Some key metabolites involved in photosynthesis such as ribulose 1,5-disphosphate, fructose 1,6-diphosphate, glycine, betaine, GABA and serine were found to be susceptible to environmental conditions. This study shows that the metabolic profiling based on CE-MS can clearly discriminate tobacco leaves of different geographical origins and understand the relationship between plant metabolites and their geographical origins.
Language英语
WOS IDWOS:000341422600004
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144497
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Jieyu,Hu, Chunxiu,Zeng, Jun,et al. Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry[J]. METABOLOMICS,2014,10(5):805-815.
APA Zhao, Jieyu.,Hu, Chunxiu.,Zeng, Jun.,Zhao, Yanni.,Zhang, Junjie.,...&许国旺.(2014).Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry.METABOLOMICS,10(5),805-815.
MLA Zhao, Jieyu,et al."Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry".METABOLOMICS 10.5(2014):805-815.
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