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学科主题: 物理化学
题名: Improved islet purity by the hypertonic-hypotonic method
作者: Li, Na1, 2, 3;  Zhang, Ying1;  Liu, Yang1;  Xiu, Zhilong2;  Tan, Mingqian1;  Li, Shen1, 2;  Guo, Xin1;  Wang, Yu1, 3;  Chen, Li1, 3;  Song, Yizhe1, 3;  Yu, Weiting1;  Lv, Guojun1;  Ma, Xiaojun1
通讯作者: 张英
关键词: Islets isolation ;  Purification ;  Hypertonic-hypotonic method ;  Plackett-Burman method ;  Density gradient separation
刊名: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
发表日期: 2014-06-01
DOI: 10.5301/ijao.5000335
卷: 37, 期:6, 页:477-485
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology ;  Life Sciences & Biomedicine
类目[WOS]: Engineering, Biomedical ;  Transplantation
研究领域[WOS]: Engineering ;  Transplantation
英文摘要: Introduction: Islet purification is usually performed using the density gradient separation method, but the purity of islets is low because exocrine cells and the embedded islets are hard to remove by using only the density gradient method. The aim of this study was to establish a new islet purification process comprising a hypertonic-hypotonic treatment step followed by a density gradient centrifugation step to improve the purity of islets.
关键词[WOS]: CELL TRANSPLANTATION ;  PANCREATIC-ISLETS ;  PURIFICATION ;  OPTIMIZATION ;  OSMOLALITY ;  LANGERHANS ;  IODIXANOL ;  SURVIVAL ;  DENSITY ;  FICOLL
语种: 英语
WOS记录号: WOS:000343850600006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144518
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Sch Life Sci & Biotechnol, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China

Recommended Citation:
Li, Na,Zhang, Ying,Liu, Yang,et al. Improved islet purity by the hypertonic-hypotonic method[J]. INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS,2014,37(6):477-485.
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