DICP OpenIR
Subject Area物理化学
Improved islet purity by the hypertonic-hypotonic method
Li, Na1,2,3; Zhang, Ying1; Liu, Yang1; Xiu, Zhilong2; Tan, Mingqian1; Li, Shen1,2; Guo, Xin1; Wang, Yu1,3; Chen, Li1,3; Song, Yizhe1,3; Yu, Weiting1; Lv, Guojun1; Ma, Xiaojun1; Zhang Y(张英)
KeywordIslets Isolation Purification Hypertonic-hypotonic Method Plackett-burman Method Density Gradient Separation
Source PublicationINTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
2014-06-01
DOI10.5301/ijao.5000335
Volume37Issue:6Pages:477-485
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology ; Life Sciences & Biomedicine
WOS SubjectEngineering, Biomedical ; Transplantation
WOS Research AreaEngineering ; Transplantation
WOS KeywordCELL TRANSPLANTATION ; PANCREATIC-ISLETS ; PURIFICATION ; OPTIMIZATION ; OSMOLALITY ; LANGERHANS ; IODIXANOL ; SURVIVAL ; DENSITY ; FICOLL
AbstractIntroduction: Islet purification is usually performed using the density gradient separation method, but the purity of islets is low because exocrine cells and the embedded islets are hard to remove by using only the density gradient method. The aim of this study was to establish a new islet purification process comprising a hypertonic-hypotonic treatment step followed by a density gradient centrifugation step to improve the purity of islets.
Language英语
WOS IDWOS:000343850600006
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144518
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang Y(张英)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Sch Life Sci & Biotechnol, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Li, Na,Zhang, Ying,Liu, Yang,et al. Improved islet purity by the hypertonic-hypotonic method[J]. INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS,2014,37(6):477-485.
APA Li, Na.,Zhang, Ying.,Liu, Yang.,Xiu, Zhilong.,Tan, Mingqian.,...&张英.(2014).Improved islet purity by the hypertonic-hypotonic method.INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS,37(6),477-485.
MLA Li, Na,et al."Improved islet purity by the hypertonic-hypotonic method".INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS 37.6(2014):477-485.
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