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学科主题物理化学
Improved islet purity by the hypertonic-hypotonic method
Li, Na1,2,3; Zhang, Ying1; Liu, Yang1; Xiu, Zhilong2; Tan, Mingqian1; Li, Shen1,2; Guo, Xin1; Wang, Yu1,3; Chen, Li1,3; Song, Yizhe1,3; Yu, Weiting1; Lv, Guojun1; Ma, Xiaojun1; Zhang Y(张英)
关键词Islets Isolation Purification Hypertonic-hypotonic Method Plackett-burman Method Density Gradient Separation
刊名INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
2014-06-01
DOI10.5301/ijao.5000335
37期:6页:477-485
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology ; Life Sciences & Biomedicine
类目[WOS]Engineering, Biomedical ; Transplantation
研究领域[WOS]Engineering ; Transplantation
关键词[WOS]CELL TRANSPLANTATION ; PANCREATIC-ISLETS ; PURIFICATION ; OPTIMIZATION ; OSMOLALITY ; LANGERHANS ; IODIXANOL ; SURVIVAL ; DENSITY ; FICOLL
英文摘要Introduction: Islet purification is usually performed using the density gradient separation method, but the purity of islets is low because exocrine cells and the embedded islets are hard to remove by using only the density gradient method. The aim of this study was to establish a new islet purification process comprising a hypertonic-hypotonic treatment step followed by a density gradient centrifugation step to improve the purity of islets.
语种英语
WOS记录号WOS:000343850600006
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/144518
专题中国科学院大连化学物理研究所
通讯作者Zhang Y(张英)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Sch Life Sci & Biotechnol, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
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GB/T 7714
Li, Na,Zhang, Ying,Liu, Yang,et al. Improved islet purity by the hypertonic-hypotonic method[J]. INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS,2014,37(6):477-485.
APA Li, Na.,Zhang, Ying.,Liu, Yang.,Xiu, Zhilong.,Tan, Mingqian.,...&张英.(2014).Improved islet purity by the hypertonic-hypotonic method.INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS,37(6),477-485.
MLA Li, Na,et al."Improved islet purity by the hypertonic-hypotonic method".INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS 37.6(2014):477-485.
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