中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: In Situ Sample Processing Approach (iSPA) for Comprehensive Quantitative Phosphoproteome Analysis
作者: Huang, Junfeng1, 2;  Qin, Hongqiang1;  Dong, Jing1;  Song, Chunxia1;  Bian, Yangyang1, 2;  Dong, Mingming1, 2;  Cheng, Kai1, 2;  Wang, Fangjun1;  Sun, Deguang3;  Wang, Liming3;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 叶明亮 ;  邹汉法
关键词: in situ sample processing approach ;  phosphoprotein enrichment ;  on-beads digestion ;  dimethyl labeling ;  solid phase labeling ;  phosphoproteome quantification ;  human liver ;  hepatocellular carcinoma
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2014-09-01
DOI: 10.1021/pr500454g
卷: 13, 期:9, 页:3896-3904
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Current sample preparation protocols for quantitative phosphoproteome analysis are tedious and time-consuming. Here, a facile in situ sample processing approach (iSPA) is developed by using macroporous Ti(IV)-IMAC microspheres as the preparation "beds", where all sample preparation procedures including the enrichment of phosphoproteins, tryptic digestion of proteins, enrichment, and isotope labeling of phosphopeptides are performed in situ sequentially. As a result of the in situ processing design and the seamless procedures, extra steps for desalting and buffer exchanging, which are always required in conventional approaches, are avoided, and the sample loss and contamination could be greatly reduced. Thus, better sensitivity and accuracy for the quantitative phosphoproteome analysis were obtained. This strategy was further applied to differential phosphoproteome analysis of human liver tissues with or without hepatocellular carcinoma (HCC). In total, 8548 phosphorylation sites were confidently quantified from three replicate analyses of 0.5 mg of human liver protein extracts.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  PHOSPHORYLATED PROTEINS ;  MASS-SPECTROMETRY ;  SELECTIVE ENRICHMENT ;  PHOSPHOPEPTIDES ;  QUANTIFICATION ;  LIVER ;  IDENTIFICATION ;  ADSORBENT ;  PEPTIDES
语种: 英语
WOS记录号: WOS:000341345000004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144524
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2014AwbMOTIsy3.PDF(2271KB)期刊论文作者接受稿开放获取View Download

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China

Recommended Citation:
Huang, Junfeng,Qin, Hongqiang,Dong, Jing,et al. In Situ Sample Processing Approach (iSPA) for Comprehensive Quantitative Phosphoproteome Analysis[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(9):3896-3904.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Huang, Junfeng]'s Articles
 [Qin, Hongqiang]'s Articles
 [Dong, Jing]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Huang, Junfeng]‘s Articles
 [Qin, Hongqiang]‘s Articles
 [Dong, Jing]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2014AwbMOTIsy3.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace