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学科主题: 物理化学
题名: Quantitative proteomics reveals the kinetics of trypsin-catalyzed protein digestion
作者: Pan, Yanbo1, 2;  Cheng, Kai1, 2;  Mao, Jiawei1, 2;  Liu, Fangjie1, 2;  Liu, Jing1, 2;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 叶明亮 ;  邹汉法
关键词: Trypsin ;  Protein digestion ;  Kinetics ;  Stable isotope dimethyl labeling ;  Mass spectrometry
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2014-10-01
DOI: 10.1007/s00216-014-8071-6
卷: 406, 期:25, 页:6247-6256
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Trypsin is the popular protease to digest proteins into peptides in shotgun proteomics, but few studies have attempted to systematically investigate the kinetics of trypsin-catalyzed protein digestion in proteome samples. In this study, we applied quantitative proteomics via triplex stable isotope dimethyl labeling to investigate the kinetics of trypsin-catalyzed cleavage. It was found that trypsin cleaves the C-terminal to lysine (K) and arginine (R) residues with higher rates for R. And the cleavage sites surrounded by neutral residues could be quickly cut, while those with neighboring charged residues (D/E/K/R) or proline residue (P) could be slowly cut. In a proteome sample, a huge number of proteins with different physical chemical properties coexists. If any type of protein could be preferably digested, then limited digestion could be applied to reduce the sample complexity. However, we found that protein abundance and other physicochemical properties, such as molecular weight (Mw), grand average of hydropathicity (GRAVY), aliphatic index, and isoelectric point (pI) have no notable correlation with digestion priority of proteins.
关键词[WOS]: MISSED CLEAVAGE SITES ;  MASS-SPECTROMETRY ;  LABEL-FREE ;  SHOTGUN PROTEOMICS ;  PHOSPHOPROTEOME ;  QUANTIFICATION ;  IDENTIFICATION ;  HYDROLYSIS ;  THROUGHPUT ;  PREDICTION
语种: 英语
WOS记录号: WOS:000342224300016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144525
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Pan, Yanbo,Cheng, Kai,Mao, Jiawei,et al. Quantitative proteomics reveals the kinetics of trypsin-catalyzed protein digestion[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2014,406(25):6247-6256.
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