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学科主题: 物理化学
题名: Effective 2D-RPLC/RPLC enrichment and separation of micro-components from Hedyotis diffusa Willd. and characterization by using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry
作者: Li, Cunman1;  Zhao, Yanyan2;  Guo, Zhimou3;  Zhang, Xiuli3;  Xue, Xingya3;  Liang, Xinmiao3
通讯作者: 梁鑫淼
关键词: Micro-component ;  UPLC-DAD/Q-TOF MS ;  2D-RPLC/RPLC ;  Click OEG stationary phase ;  Hedyotis diffusa Willd
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2014-10-01
DOI: 10.1016/j.jpba.2014.06.020
卷: 99, 期:1, 页:35-44
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: An effective method aiming at enrichment and analysis of micro-components in traditional Chinese medicine (TCM) was developed. One fraction (fraction E) from the extract of Hedyotis diffusa Willd. was selected as test sample, which was isolated by using the XAD-4 macroporous resin. To study the micro-components, a two-dimensional reverse-phase liquid chromatography (2D-RPLC/RPLC) method was developed, comprising Click OEG and C18 stationary phases as the first and second dimensions, respectively. Of the eight sub-fractions isolated from the first dimension, three sub-fractions (fractions II-IV) containing micro-components were further separated with the second dimension. The 2D-RPLC/RPLC system was proved to possess high orthogonality. Furthermore, the micro-components were characterized by using ultra-performance liquid chromatography-diode array detector/quadrupole time-of-flight mass spectrometry (UPLC-DAD/Q-TOF MS) with electrospray ionization (ESI) source. With the optimized separation and characterization method, a large number (>400) of micro-components were enriched and detected from the extracts of H. diffusa Willd., the majority of which has not been isolated from the herb before. Among these isolated micro-components, 38 compounds involving 24 phenylpropanoids, 7 flavonoids and 7 iridoid glucosides (IGs), were identified or tentatively identified from the H. diffusa extracts on the basis of spectral data of the authentic standards and the fragmentation characteristics information available in literatures. The proposed method made it possible to effectively screen and analyze the micro-components in TCMs or other complex natural medicines. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: TRADITIONAL CHINESE MEDICINE ;  IRIDOID GLUCOSIDES ;  ELECTROSPRAY-IONIZATION ;  OLIGO(ETHYLENE GLYCOL) ;  STATIONARY-PHASE ;  CLICK ;  CONSTITUENTS ;  GLYCOSIDES ;  CORYMBOSA ;  COLUMN
语种: 英语
WOS记录号: WOS:000341169100006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144526
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Hebei Univ Sci & Technol, Hebei Res Ctr Anal & Testing, Shijiazhuang 050018, Peoples R China
2.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Li, Cunman,Zhao, Yanyan,Guo, Zhimou,et al. Effective 2D-RPLC/RPLC enrichment and separation of micro-components from Hedyotis diffusa Willd. and characterization by using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,99(1):35-44.
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