Subject Area物理化学
等重二甲基化标记蛋白质定量方法; 等重二甲基化标记蛋白质定量方法; 等重二甲基化标记蛋白质定量方法; 等重二甲基化标记蛋白质定量方法
张丽华; 周愿; 单亦初; 杨开广; 吴琪; 张珅; 张玉奎
Application NumberCN201310106708.0
Patent NumberCN201310106708.0
Application Date2013-03-29
Rights Holder中国科学院大连化学物理研究所
PCT Attributes
Copyright Date2015-08-19
等重二甲基化标记蛋白质定量方法; 等重二甲基化标记蛋白质定量方法; 等重二甲基化标记蛋白质定量方法; 等重二甲基化标记蛋白质定量方法
Date Available2015-08-19
Other AbstractThe invention relates to a new protein quantitative method, in particular to a quantitative method utilizing equiponderance dimethylation marking. The method utilizes the organic combination of methanal and isotope of the methanal, and sodium cyanoborohydride and isotope of the sodium cyanoborohydride for marking peptide fragments, so that the peptide fragments have the similar mass-to-charge ratio on the primary spectrum of the mass spectrum and have the different mass-to-charge ratios on the secondary spectrum of the mass spectrum, and the protein quantitative analysis is achieved on the secondary spectrum. Compared with other quantitative methods, the method has the advantages of being high in quantitative accuracy and precision, and wide in dynamic range. | 本发明涉及了一种新的蛋白质定量方法-等重二甲基化标记定量法,该方法利用甲醛和氰基硼氢化钠及其各自的同位素形式进行有机组合,对肽段进行标记,使得肽段在质谱的一级谱上具有相近质荷比而在二级谱上具有不同质荷比,从而在二级谱上实现蛋白质定量分析。该方法相较于其他定量方法,该方法具有定量准确度高,精密度高和动态范围高等优点。
Document Type专利
Recommended Citation
GB/T 7714
张丽华,周愿,单亦初,等. 等重二甲基化标记蛋白质定量方法, 等重二甲基化标记蛋白质定量方法, 等重二甲基化标记蛋白质定量方法, 等重二甲基化标记蛋白质定量方法. CN201310106708.0[P]. 2015-08-19.
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