Subject Area物理化学
HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法; HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法; HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法; HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法
张保琴; 张海军; 金静; 陈吉平
Application NumberCN201210085037.X
Patent NumberCN201210085037.X
Application Date2012-03-27
Rights Holder中国科学院大连化学物理研究所
PCT Attributes
Copyright Date2015-01-07
HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法; HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法; HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法; HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法
Date Available2015-01-07
Other AbstractThe invention relates to a method for evaluating the cytotoxicity of environmental pollutants. The method adopts a HepG2 cell as a test cell and comprises the following steps: (a) establishing a pretreatment method for extracting metabolites from a cell culture fluid rapidly; (b) analyzing the 23 metabolites in the cell culture fluid quantitatively by adopting HPLC-MS/MS (high performance liquid chromatography and mass spectrometry or mass spectrometry); (c) establishing the metabolic profile chart of the HepG2 cell; and (d) analyzing the influence of the environmental pollutants on all the metabolic pathways of the HepG2 cell successfully by adopting a metabolic flux analysis system according to data obtained by adopting the HPLC-MS/MS. The pretreatment method is simple, various metabolites in the cell culture fluid can be quantitated accurately by adopting the HPLC-MS/MS, and the influence of the environmental pollutants on the metabolic pathways of the HepG2 cell can be reflected intuitively by combining the metabolic flux analysis system. The method provided by the invention has the advantages of simple operation, short analysis time, high sensitivity, good reproducibility and the like, and comprehensive information can be obtained. | 本发明利用HepG2细胞为供试细胞,a)建立了细胞培养液中快速提取代谢产物的前处理方法;b)利用HPLC-MS/MS定量分析细胞培养液中的23种代谢物;c)构建了HepG2细胞的代谢轮廓;d)代谢流量分析系统利用HPLC-MS/MS所得数据成功分析环境污染物对细胞各代谢路径的影响。该发明前处理方法简单,利用HPLC-MS/MS能精确定量细胞培养液中的多种代谢物,结合代谢流量分析系统能直观的反映环境污染物对细胞代谢路径的影响;具有操作简单,分析时间短,灵敏度高,重现性好,获得信息量全面等优点。
Document Type专利
Recommended Citation
GB/T 7714
张保琴,张海军,金静,等. HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法, HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法, HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法, HPLC-MS/MS结合代谢流量分析系统评估环境污染物细胞毒性的方法. CN201210085037.X[P]. 2015-01-07.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[张保琴]'s Articles
[张海军]'s Articles
[金静]'s Articles
Baidu academic
Similar articles in Baidu academic
[张保琴]'s Articles
[张海军]'s Articles
[金静]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[张保琴]'s Articles
[张海军]'s Articles
[金静]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.