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题名: A novel approach for fluorescent visualization of glycyrrhetic acid on a cell with a quantum dot
作者: Hou, Jie1, 2;  Shi, Qian2;  Cao, Meirong2;  Pan, Pengwei2;  Ge, Guangbo3;  Fan, Xuran3;  Bai, Gang2;  Xin, Yi1
关键词: fluorescent visualization ;  glycyrrhetic acid ;  quantum dot ;  click reaction
刊名: BIOCHEMISTRY-MOSCOW
发表日期: 2014
DOI: 10.1134/S0006297914010040
卷: 79, 期:1, 页:25-30
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Glycyrrhetic acid (GA), a pentacyclic triterpenoid derivative obtained from hydrolysis of glycyrrhizic acid, was found to have synergistic anti-asthmatic effects with the beta(2)-adrenergic receptor (beta(2)AR) agonist via the beta(2)AR-mediated pathway. This study visualized the location of GA on a human cell expressing beta(2)AR via chemical biological approaches. A CdTe/ZnS quantum dot modified with an alkynyl group (QD-AL) was first synthesized, and an azide-terminal GA (ATGA) was also prepared. The QD-AL was used for fluorescence visualization of the distribution of GA on human embryonic kidney 293 cells expressing fusion beta(2)AR (HEK293-beta(2)AR) through the "click reaction" between QD-AL and ATGA. The average size of the QD-AL nanoparticle was about 10 nm, and its fluorescent emission wavelength was 620 nm. The location of GA on the HEK293-beta(2)AR cell membrane can be visualized by the click reaction (between QD-AL and ATGA). The ability of QD-AL targeting to ATGA on the cell membrane of a HEK293-beta(2)AR cell was further investigated using both confocal laser-scanning microscopy and a cellular uptake-inhibition assay. The results reveal that QD-AL can recognize ATGA on the cell membrane through the click reaction, which provides a new approach for visualizing the location of GA on the cell in an indirect way, and it can be applied to explore the synergistic anti-asthmatic mechanism of GA with beta(2)AR agonist through the beta(2)AR mediated pathway.
关键词[WOS]: RECEPTOR AGONIST ;  NANOCRYSTALS ;  PHASE
语种: 英语
WOS记录号: WOS:000330962200004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145412
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Dept Biochem & Mol Biol, Dalian 116044, Peoples R China
2.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China

Recommended Citation:
Hou, Jie,Shi, Qian,Cao, Meirong,et al. A novel approach for fluorescent visualization of glycyrrhetic acid on a cell with a quantum dot[J]. BIOCHEMISTRY-MOSCOW,2014,79(1):25-30.
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