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题名: Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection
作者: Tang, Chunlan1;  Wang, Li1;  Cheng, Mengchun1;  Zhang, Xiaozhe1;  Liu, Xiaoyan1;  Xiao, Hongbin1
关键词: Parishin ;  UHPLC-FLD ;  Metabolites ;  Pharmacokinetics
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2014-12-15
DOI: 10.1016/j.jchromb.2014.08.020
卷: 973, 页:104-109
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A simple, rapid and sensitive ultra high performance liquid chromatography with fluorescence detection (UHPLC-FLD) method was developed and validated for quantification of parishin and its metabolites in rats. Plasma samples were prepared by protein precipitation and then analyzed using UHPLC-FLD system. Repeated optimization showed that parishin and its metabolites, including gastrodin, p-hydroxybenzyl alcohol, parishin B and parishin C, could be sensitively detected based on the autofluorescence when excitation and emission wavelengths were set at 225 nm and 295 nm, respectively. The limit of detections (LODs) of GAS, HBA, PB, PC and PA reached 0.6, 0.8, 1, 1 and 1 ng/mL, respectively. The linearity for all targets was within the range 2.5-5000 ng/mL and the correlation coefficient (r(2)) was larger than 0.999. Importantly, our method was almost free from matrix effects and the recoveries were higher than 80%. Additionally, our method also had high precision and accuracy for all analytes, presenting RSDs and REs within +/- 6% and +/- 14%, respectively. Finally, the validated UHPLC-FLD method was successfully applied for studying the pharmacokinetics of parishin following intragastrically administration in rats. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: GASTRODIA-ELATA-BL. ;  PHARMACOKINETICS ;  IDENTIFICATION ;  MICRODIALYSIS ;  CONSTITUENTS ;  RHIZOMES
语种: 英语
WOS记录号: WOS:000345878800015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145414
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Tang, Chunlan,Wang, Li,Cheng, Mengchun,et al. Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2014,973:104-109.
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