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题名: New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC
作者: Wang, Lina1;  Quan, Chunshan2, 3;  Xiong, Wen2, 3;  Qu, Xiaojing2, 3;  Fan, Shengdi2, 3;  Hu, Wenzhong2, 3
关键词: AgrC ;  Topology ;  GFP ;  SCAM ;  Staphylococcus aureus
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
发表日期: 2014-03-01
DOI: 10.1016/j.bbamem.2013.12.006
卷: 1838, 期:3, 页:988-993
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics
英文摘要: Staphylococcus aureus accessory gene regulator (agr) locus controls the expression of virulence factors through a classical two-component signal transduction system that consists of a receptor histidine protein kinase AgrC and a cytoplasmic response regulator AgrA. An autoinducing peptide (AIP) encoded by agr locus activates AgrC, which transduces extracellular signals into the cytoplasm. Despite extensive investigations to identify AgrC-AIP interaction sites, precise signal recognition mechanisms remain unknown. This study aims to clarify the membrane topology of AgrC by applying the green fluorescent protein (GFP) fusion technique and the substituted cysteine accessibility method (SCAM). However, our findings were inconsistent with profile obtained previously by alkaline phosphatase. We report the topology of AgrC shows seven transmembrane segments, a periplasmic N-terminus, and a cytoplasmic C-terminus. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: CYSTEINE ACCESSIBILITY METHOD ;  MEMBRANE-PROTEIN TOPOGENESIS ;  VIRULENCE GENES ;  REGULATORY RNA ;  UNITED-STATES ;  RF CLONING ;  RECEPTOR ;  PREDICTION ;  RECOGNITION ;  IDENTIFICATION
语种: 英语
WOS记录号: WOS:000331661800029
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145426
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Nationalities Univ, Dept Life Sci, Dalian 116600, Peoples R China
3.State Ethn Affairs Commiss, Minist Educ Econ & Technol Dev Zone, Dalian 116600, Peoples R China

Recommended Citation:
Wang, Lina,Quan, Chunshan,Xiong, Wen,et al. New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,2014,1838(3):988-993.
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