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New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC
Wang, Lina1; Quan, Chunshan2,3; Xiong, Wen2,3; Qu, Xiaojing2,3; Fan, Shengdi2,3; Hu, Wenzhong2,3
KeywordAgrc Topology Gfp Scam Staphylococcus Aureus
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
2014-03-01
DOI10.1016/j.bbamem.2013.12.006
Volume1838Issue:3Pages:988-993
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS KeywordCYSTEINE ACCESSIBILITY METHOD ; MEMBRANE-PROTEIN TOPOGENESIS ; VIRULENCE GENES ; REGULATORY RNA ; UNITED-STATES ; RF CLONING ; RECEPTOR ; PREDICTION ; RECOGNITION ; IDENTIFICATION
AbstractStaphylococcus aureus accessory gene regulator (agr) locus controls the expression of virulence factors through a classical two-component signal transduction system that consists of a receptor histidine protein kinase AgrC and a cytoplasmic response regulator AgrA. An autoinducing peptide (AIP) encoded by agr locus activates AgrC, which transduces extracellular signals into the cytoplasm. Despite extensive investigations to identify AgrC-AIP interaction sites, precise signal recognition mechanisms remain unknown. This study aims to clarify the membrane topology of AgrC by applying the green fluorescent protein (GFP) fusion technique and the substituted cysteine accessibility method (SCAM). However, our findings were inconsistent with profile obtained previously by alkaline phosphatase. We report the topology of AgrC shows seven transmembrane segments, a periplasmic N-terminus, and a cytoplasmic C-terminus. (C) 2013 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000331661800029
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145426
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Nationalities Univ, Dept Life Sci, Dalian 116600, Peoples R China
3.State Ethn Affairs Commiss, Minist Educ Econ & Technol Dev Zone, Dalian 116600, Peoples R China
Recommended Citation
GB/T 7714
Wang, Lina,Quan, Chunshan,Xiong, Wen,et al. New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,2014,1838(3):988-993.
APA Wang, Lina,Quan, Chunshan,Xiong, Wen,Qu, Xiaojing,Fan, Shengdi,&Hu, Wenzhong.(2014).New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC.BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,1838(3),988-993.
MLA Wang, Lina,et al."New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC".BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1838.3(2014):988-993.
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