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题名: Highly Efficient Preparation of Bisphenol A Glucuronide
作者: Yang Gang1;  Zhu Liangliang2;  Lu Xia2;  Wu Dachang1;  Xia Yangliu2;  Ma Xiaochi1;  Xin Yi1;  Hou Jie1
关键词: Bisphenol A ;  Rat liver microsome ;  Bisphenol A-beta-O-glucuronide ;  Solid-phase extraction
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2014-02-10
DOI: 10.7503/cjcu20130613
卷: 35, 期:2, 页:314-318
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Bisphenol A(BPA), an industrial chemical, has been present in many hard plastic bottles, metal-based food and beverage cans. Many governments are trying to reduce the human exposure to BPA because it is really a readily accessible toxin for human. BPA can be extensively metabolized to bisphenol A-beta-O-glucuronide(BPA-G) by UDP-glucuronosyltransferases(UGTs), thus the biological activities of BPA-G should be studied intensively. However, the studies on BPA-G are very limited due to the difficulty for preparation of BPA-G. In this work, the BPA-G was prepared using BPA as the starting material and rat liver microsomes (RLM) as the enzyme source. A series of orthogonal experiments was designed and performed to determine. the optimal reaction conditions for BPA glucuronidation in RLM. After incubation under the optimal reaction conditions, the conversion of BPA was higher than 98%. A unique solid-phase extraction column (SPE) packed with C-18 WAX was used to enrich and purify the product BPA-G with high yield. Finally, the product was identified by both ESI-MS and NMR techniques.
关键词[WOS]: MASS-SPECTROMETRY ;  LIVER ;  DIETHYLSTILBESTROL ;  INTESTINE ;  ISOFORMS ;  EXPOSURE ;  ADULT
语种: 英语
WOS记录号: WOS:000332918400018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145457
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Dept Biotechnol, Dalian 116044, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Yang Gang,Zhu Liangliang,Lu Xia,et al. Highly Efficient Preparation of Bisphenol A Glucuronide[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2014,35(2):314-318.
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