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题名: Effect of Different Charged Liposomes on Transmembrane Incorporation Efficiency of the Staphylococcus Aureus Receptor Histidine Kinase AgrC
作者: Xiong Wen1, 2, 3;  Quan Chunshan1, 2, 3;  Wang Lina4;  Zhang Xuning5;  Zhao Jing1, 2, 3;  Zheng Wei1, 2, 3;  Fan Shengdi1, 2, 3
关键词: Staphylococcus aureus ;  AgrC ;  Liposome ;  Proteoliposome ;  Membrane protein
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2014-09-10
DOI: 10.7503/cjcu20140057
卷: 35, 期:9, 页:1901-1907
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: AgrC is a membrane-embedded histidine kinase in Staphylococcus aureus that is thought to act as a sensor for the recognition of environmental signals and the transduction of signals into the cytoplasm so as to regulate and control a series of related pathogenic gene expression. However, for the complexity of cell membrane, it turns to be difficult to study AgrC on bacterial cell membrane directly. Many researches tried to take advantage of proteoliposome which could provide an approximate natural membrane environment and keep the protein activity to study the structure and function of membrane proteins. In order to build a transmembrane protein incorporation system with a relatively high incorporation efficiency, function activity and stability, various factors were considered, such as the impact of different charge polarity head on protein transmembrane incorporation efficiency. Here, four kinds of different charged liposomes were prepared by changing the phospholipid components in liposome and a detergent-mediated method was used to reconstitute truncated AgrC protein(AgrC(TM6-7C)) into them. The results showed that negatively charged liposomes leaded to a higher incorporation efficiency compared with the positively charged one and about 60%-70% C-terminal cytoplasmic domain of AgrC(TM6-7C) c protein reconstituted into negatively charged liposomes were outside-orientated which remained a highly kinase activity. Furthermore, from circular dichroism detection result, we can draw a conclusion that liposome has certain protective effect on AgrC(TM6-7C)-protein secondary structure and improve the thermal stability of the protein.
关键词[WOS]: LIPID-PROTEIN INTERACTIONS ;  MEMBRANE-PROTEINS ;  VIRULENCE ;  PERSPECTIVE ;  INFECTIONS ;  SENSOR
语种: 英语
WOS记录号: WOS:000343189300009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145470
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Nationalities Univ, Key Lab Biotechnol & Resource Utilizat, State Ethn Affairs Commiss, Dalian 116600, Peoples R China
2.Dalian Nationalities Univ, Minist Educ, Dalian 116600, Peoples R China
3.Dalian Nationalities Univ, Dept Life Sci, Dalian 116600, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
5.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China

Recommended Citation:
Xiong Wen,Quan Chunshan,Wang Lina,et al. Effect of Different Charged Liposomes on Transmembrane Incorporation Efficiency of the Staphylococcus Aureus Receptor Histidine Kinase AgrC[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2014,35(9):1901-1907.
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