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题名: Hydrophilic interaction liquid chromatography-solid phase extraction directly combined with protein precipitation for the determination of triptorelin in plasma
作者: Wang, Jixia1;  Kong, Song2;  Yan, Jingyu1;  Jin, Gaowa1;  Guo, Zhimou1;  Shen, Aijin1;  Xu, Junyan1;  Zhang, Xiuli1;  Zou, Lijuan2;  Liang, Xinmiao1
关键词: HILIC-SPE ;  PPT ;  RPLC-MS/MS ;  Triptorelin ;  Plasma
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2014-06-01
DOI: 10.1016/j.jchromb.2014.04.036
卷: 960, 页:214-221
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Peptide drugs play a critical role in therapeutic treatment. However, as the complexity of plasma, determination of peptide drugs using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a daunting task. To solve this problem, hydrophilic interaction liquid chromatography-solid phase extraction (HILIC-SPE) directly combined with protein precipitation (PPT) was developed for the selective extraction of triptorelin from plasma. The extracts were analyzed by reversed-phase liquid chromatography (RPLC). Proteins, phospholipids and highly polar interferences could be removed from plasma by the efficient combination of PPT, HILIC-SPE and RPLC-MS/MS. This method was evaluated by matrix effect, recovery and process efficiency at different concentration levels (50, 500 and 5000 ng/mL) of triptorelin. Furthermore, the performance of HILIC-SPE was compared with that of reversed-phase C18 SPE and hydrophilic lipophilic balance (Oasis HLB) SPE. Among them, HILIC-SPE provided the minimum matrix effect (ranging from 96.02% to 103.41%), the maximum recovery (ranging from 80.68% to 90.54%) and the satisfactory process efficiency (ranging from 82.83% to 92.95%). The validated method was successfully applied to determine triptorelin in rat plasma. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  SAMPLE PREPARATION ;  REVERSED-PHASE ;  ELECTROSPRAY-IONIZATION ;  BIOLOGICAL FLUID ;  ION SUPPRESSION ;  MATRIX ;  QUANTIFICATION ;  PEPTIDES ;  PHOSPHOLIPIDS
语种: 英语
WOS记录号: WOS:000336821600030
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145478
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dept Radiat Oncol, Dalian 116027, Peoples R China

Recommended Citation:
Wang, Jixia,Kong, Song,Yan, Jingyu,et al. Hydrophilic interaction liquid chromatography-solid phase extraction directly combined with protein precipitation for the determination of triptorelin in plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2014,960:214-221.
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