DICP OpenIR
Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells
Song, Huiyi1,3; Yu, Weiting1; Liu, Xiudong2; Ma, Xiaojun1
KeywordMicroencapsulated Cell Culture Probiotic Viability Resistance To Stress Environments
Source PublicationCARBOHYDRATE POLYMERS
2014-08-08
DOI10.1016/j.carbpol.2014.02.084
Volume108Pages:10-16
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS Research AreaChemistry ; Polymer Science
WOS KeywordSIMULATED GASTROINTESTINAL CONDITIONS ; LACTIC-ACID BACTERIA ; LACTOBACILLUS-ACIDOPHILUS ; SURVIVAL ; STORAGE ; ENCAPSULATION ; STABILITY ; CRYOPROTECTANTS ; BIFIDOBACTERIUM ; MICROSPHERES
AbstractIn this study, probiotics (Saccharomyces cerevisiae Y235) were entrapped in alginate-chitosan microcapsules by emulsification/internal gelation technique. Two different encapsulation patterns were established as directly entrapped high density cells (dEHDC) and entrapped low density cells with culture (ELDCwc). The performance of microencapsulated cells, with free cells (FC) as control, was investigated against sequential stress environments of freeze-drying, storage, and simulated gastrointestinal fluids. After being freeze-dried without cryoprotectant, the survival rate of ELDCwc (14.33%) was significantly higher than 10.00% of dEHDC, and 0.05% of FC. The lower temperature (-20 degrees C) and ELDCwc pattern were beneficial for keeping viable cells at 7.00 log CFU g-(1) after 6 months. Furthermore, the ELDCwc microcapsule maintained viable cells of 6.29 log CFU g(-1) after incubation in SGF and SIF. These studies demonstrated that the pattern of entrapped low density cells with culture was an effective and superior technique of resisting harmful stress environments. (c) 2014 Elsevier Ltd. All rights reserved.
Language英语
WOS IDWOS:000336193600002
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145487
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, PR, Peoples R China
2.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Song, Huiyi,Yu, Weiting,Liu, Xiudong,et al. Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells[J]. CARBOHYDRATE POLYMERS,2014,108:10-16.
APA Song, Huiyi,Yu, Weiting,Liu, Xiudong,&Ma, Xiaojun.(2014).Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells.CARBOHYDRATE POLYMERS,108,10-16.
MLA Song, Huiyi,et al."Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells".CARBOHYDRATE POLYMERS 108(2014):10-16.
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