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题名: Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells
作者: Song, Huiyi1, 3;  Yu, Weiting1;  Liu, Xiudong2;  Ma, Xiaojun1
关键词: Microencapsulated cell culture ;  Probiotic viability ;  Resistance to stress environments
刊名: CARBOHYDRATE POLYMERS
发表日期: 2014-08-08
DOI: 10.1016/j.carbpol.2014.02.084
卷: 108, 页:10-16
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Applied ;  Chemistry, Organic ;  Polymer Science
研究领域[WOS]: Chemistry ;  Polymer Science
英文摘要: In this study, probiotics (Saccharomyces cerevisiae Y235) were entrapped in alginate-chitosan microcapsules by emulsification/internal gelation technique. Two different encapsulation patterns were established as directly entrapped high density cells (dEHDC) and entrapped low density cells with culture (ELDCwc). The performance of microencapsulated cells, with free cells (FC) as control, was investigated against sequential stress environments of freeze-drying, storage, and simulated gastrointestinal fluids. After being freeze-dried without cryoprotectant, the survival rate of ELDCwc (14.33%) was significantly higher than 10.00% of dEHDC, and 0.05% of FC. The lower temperature (-20 degrees C) and ELDCwc pattern were beneficial for keeping viable cells at 7.00 log CFU g-(1) after 6 months. Furthermore, the ELDCwc microcapsule maintained viable cells of 6.29 log CFU g(-1) after incubation in SGF and SIF. These studies demonstrated that the pattern of entrapped low density cells with culture was an effective and superior technique of resisting harmful stress environments. (c) 2014 Elsevier Ltd. All rights reserved.
关键词[WOS]: SIMULATED GASTROINTESTINAL CONDITIONS ;  LACTIC-ACID BACTERIA ;  LACTOBACILLUS-ACIDOPHILUS ;  SURVIVAL ;  STORAGE ;  ENCAPSULATION ;  STABILITY ;  CRYOPROTECTANTS ;  BIFIDOBACTERIUM ;  MICROSPHERES
语种: 英语
WOS记录号: WOS:000336193600002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145487
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, PR, Peoples R China
2.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Song, Huiyi,Yu, Weiting,Liu, Xiudong,et al. Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells[J]. CARBOHYDRATE POLYMERS,2014,108:10-16.
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