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Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells
Song, Huiyi1,3; Yu, Weiting1; Liu, Xiudong2; Ma, Xiaojun1
关键词Microencapsulated Cell Culture Probiotic Viability Resistance To Stress Environments
刊名CARBOHYDRATE POLYMERS
2014-08-08
DOI10.1016/j.carbpol.2014.02.084
108页:10-16
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]Chemistry ; Polymer Science
关键词[WOS]SIMULATED GASTROINTESTINAL CONDITIONS ; LACTIC-ACID BACTERIA ; LACTOBACILLUS-ACIDOPHILUS ; SURVIVAL ; STORAGE ; ENCAPSULATION ; STABILITY ; CRYOPROTECTANTS ; BIFIDOBACTERIUM ; MICROSPHERES
英文摘要In this study, probiotics (Saccharomyces cerevisiae Y235) were entrapped in alginate-chitosan microcapsules by emulsification/internal gelation technique. Two different encapsulation patterns were established as directly entrapped high density cells (dEHDC) and entrapped low density cells with culture (ELDCwc). The performance of microencapsulated cells, with free cells (FC) as control, was investigated against sequential stress environments of freeze-drying, storage, and simulated gastrointestinal fluids. After being freeze-dried without cryoprotectant, the survival rate of ELDCwc (14.33%) was significantly higher than 10.00% of dEHDC, and 0.05% of FC. The lower temperature (-20 degrees C) and ELDCwc pattern were beneficial for keeping viable cells at 7.00 log CFU g-(1) after 6 months. Furthermore, the ELDCwc microcapsule maintained viable cells of 6.29 log CFU g(-1) after incubation in SGF and SIF. These studies demonstrated that the pattern of entrapped low density cells with culture was an effective and superior technique of resisting harmful stress environments. (c) 2014 Elsevier Ltd. All rights reserved.
语种英语
WOS记录号WOS:000336193600002
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145487
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, PR, Peoples R China
2.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Song, Huiyi,Yu, Weiting,Liu, Xiudong,et al. Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells[J]. CARBOHYDRATE POLYMERS,2014,108:10-16.
APA Song, Huiyi,Yu, Weiting,Liu, Xiudong,&Ma, Xiaojun.(2014).Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells.CARBOHYDRATE POLYMERS,108,10-16.
MLA Song, Huiyi,et al."Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells".CARBOHYDRATE POLYMERS 108(2014):10-16.
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