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题名: A novel method for characterization and comparison of reversed-phase column selectivity
作者: Wang, Jixia1;  Wang, Chaoran1;  Guo, Zhimou1;  Dong, Xuefang1;  Xiao, Yuansheng1;  Xue, Xingya1;  Zhang, Xiuli1;  Liang, Xinmiao1
关键词: Reversed-phase column selectivity ;  LSERs ;  Fundamental retention equations ;  CSASS ;  Three linear gradient elutions
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-09-26
DOI: 10.1016/j.chroma.2014.08.005
卷: 1361, 页:153-161
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Characterization of reverse-phase column selectivity is helpful for chromatographers to select an optimal column. A novel method, based on linear solvation energy relationships (LSERs) combined with fundamental retention equations, was developed to characterize and compare reversed-phase column selectivity. The retention times of 25 elaborately selected solutes on 12 reversed-phase columns were determined in three linear gradient elutions. Using these retention times, fundamental retention equations were acquired by a complex sample analysis software system (CSASS). When 0%, 10%, 20%, 30%, 40% and 50% acetonitrile were introduced into the fundamental retention equations, the corresponding retention factors were predicted and used to obtain LSER equations by multiple linear regression. In the gradient elution, the retention times of solutes could be accurately determined and the excessively long or short analysis time could be avoided. As the retention factor (ln k(w)) at a hypothetical 0% organic modifier closely reflected properties of columns, coefficients of LSERs equations obtained based on In kw were employed to discuss the properties of different stationary phases. An angle and a spider diagram based on solvation energy vectors were used to compare selectivity differences between stationary phases, which provided a visual means for users to select appropriate columns with orthogonal or similar selectivity. These results of column selectivities were compared with those obtained by geometric orthogonality approach, and a consistent result was acquired. Finally, Click TE-CD and XCharge C18PN with highest difference in column selectivity were applied to the separation of Psoralea cotylifolia extraction. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  SOLVATION ENERGY RELATIONSHIPS ;  STRUCTURE-RETENTION RELATIONSHIPS ;  SUPERCRITICAL-FLUID CHROMATOGRAPHY ;  SOLVATOCHROMIC COMPARISON METHOD ;  STATIONARY PHASES ;  GRADIENT ELUTION ;  MOLECULAR-MECHANISM ;  CHEMOMETRIC METHODS ;  PARAMETER MODEL
语种: 英语
WOS记录号: WOS:000342257200018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145496
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Jixia,Wang, Chaoran,Guo, Zhimou,et al. A novel method for characterization and comparison of reversed-phase column selectivity[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1361:153-161.
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