DICP OpenIR
A novel method for characterization and comparison of reversed-phase column selectivity
Wang, Jixia; Wang, Chaoran; Guo, Zhimou; Dong, Xuefang; Xiao, Yuansheng; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao
KeywordReversed-phase Column Selectivity Lsers Fundamental Retention Equations Csass Three Linear Gradient Elutions
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2014-09-26
DOI10.1016/j.chroma.2014.08.005
Volume1361Pages:153-161
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; SOLVATION ENERGY RELATIONSHIPS ; STRUCTURE-RETENTION RELATIONSHIPS ; SUPERCRITICAL-FLUID CHROMATOGRAPHY ; SOLVATOCHROMIC COMPARISON METHOD ; STATIONARY PHASES ; GRADIENT ELUTION ; MOLECULAR-MECHANISM ; CHEMOMETRIC METHODS ; PARAMETER MODEL
AbstractCharacterization of reverse-phase column selectivity is helpful for chromatographers to select an optimal column. A novel method, based on linear solvation energy relationships (LSERs) combined with fundamental retention equations, was developed to characterize and compare reversed-phase column selectivity. The retention times of 25 elaborately selected solutes on 12 reversed-phase columns were determined in three linear gradient elutions. Using these retention times, fundamental retention equations were acquired by a complex sample analysis software system (CSASS). When 0%, 10%, 20%, 30%, 40% and 50% acetonitrile were introduced into the fundamental retention equations, the corresponding retention factors were predicted and used to obtain LSER equations by multiple linear regression. In the gradient elution, the retention times of solutes could be accurately determined and the excessively long or short analysis time could be avoided. As the retention factor (ln k(w)) at a hypothetical 0% organic modifier closely reflected properties of columns, coefficients of LSERs equations obtained based on In kw were employed to discuss the properties of different stationary phases. An angle and a spider diagram based on solvation energy vectors were used to compare selectivity differences between stationary phases, which provided a visual means for users to select appropriate columns with orthogonal or similar selectivity. These results of column selectivities were compared with those obtained by geometric orthogonality approach, and a consistent result was acquired. Finally, Click TE-CD and XCharge C18PN with highest difference in column selectivity were applied to the separation of Psoralea cotylifolia extraction. (C) 2014 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000342257200018
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145496
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jixia,Wang, Chaoran,Guo, Zhimou,et al. A novel method for characterization and comparison of reversed-phase column selectivity[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1361:153-161.
APA Wang, Jixia.,Wang, Chaoran.,Guo, Zhimou.,Dong, Xuefang.,Xiao, Yuansheng.,...&Liang, Xinmiao.(2014).A novel method for characterization and comparison of reversed-phase column selectivity.JOURNAL OF CHROMATOGRAPHY A,1361,153-161.
MLA Wang, Jixia,et al."A novel method for characterization and comparison of reversed-phase column selectivity".JOURNAL OF CHROMATOGRAPHY A 1361(2014):153-161.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Jixia]'s Articles
[Wang, Chaoran]'s Articles
[Guo, Zhimou]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Jixia]'s Articles
[Wang, Chaoran]'s Articles
[Guo, Zhimou]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Jixia]'s Articles
[Wang, Chaoran]'s Articles
[Guo, Zhimou]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.