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A novel preparative liquid chromatograph for repetitive enrichment and purification of low-abundance compounds
Zou, Xiaowei1,2; Gao, Mingzhe1; Liu, Dan1; Zhang, Xiaozhe1; Xiu, Zhilong2; Xiao, Hongbin1,3
关键词Recycle Preparative Hplc Repetitive Enrichment Low Abundance Compound Column Capture Mobile Phase Adjusting
刊名JOURNAL OF CHROMATOGRAPHY A
2014-07-18
DOI10.1016/j.chroma.2014.05.041
1351页:90-95
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]RECYCLE CHROMATOGRAPHY ; SAUSSUREA-INVOLUCRATA ; PHASE ; INJECTION ; SOLVENTS ; DESIGN ; SYSTEM
英文摘要A novel recycle preparative HPLC system was developed to repeatedly enrich and purify low-abundance compounds from complex samples. The recycle separation involves injecting a large volume of the sample through a trap column, releasing the absorbed sample for preparative HPLC separation, and then capturing the target component in the same trap column for the next separation. After several cycles, the final purified target compound absorbed in the trap column is eluted with a small volume of solvent. The capture and recycle procedure was realized by adjusting the strength of the mobile phase using an auxiliary pump and switching the two valves connecting the trap and preparative columns. Compared with standard HPLC or existing recycle chromatography, this system not only reduces the solvent side-effects and sample volume overloading during injection but also avoids peak-broadening and sensitivity loss during the recycle runs. Moreover, the final purified products can be easily concentrated. With the aid of this system, a 10% polyphenol in extracts from Saussurea involucrata cultured cells reached >95% purity with more than 95% recovery after three recycle purifications, and a 1% unstable epimer impurity in the raw material of the drug silybin reached >95% purity with 75% recovery after a six-cycle separation. (C) 2014 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000337990400011
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145502
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dept Biosci & Biotechnol, Dalian 116024, Peoples R China
3.Chinese Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China
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GB/T 7714
Zou, Xiaowei,Gao, Mingzhe,Liu, Dan,et al. A novel preparative liquid chromatograph for repetitive enrichment and purification of low-abundance compounds[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1351:90-95.
APA Zou, Xiaowei,Gao, Mingzhe,Liu, Dan,Zhang, Xiaozhe,Xiu, Zhilong,&Xiao, Hongbin.(2014).A novel preparative liquid chromatograph for repetitive enrichment and purification of low-abundance compounds.JOURNAL OF CHROMATOGRAPHY A,1351,90-95.
MLA Zou, Xiaowei,et al."A novel preparative liquid chromatograph for repetitive enrichment and purification of low-abundance compounds".JOURNAL OF CHROMATOGRAPHY A 1351(2014):90-95.
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