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题名: A novel preparative liquid chromatograph for repetitive enrichment and purification of low-abundance compounds
作者: Zou, Xiaowei1, 2;  Gao, Mingzhe1;  Liu, Dan1;  Zhang, Xiaozhe1;  Xiu, Zhilong2;  Xiao, Hongbin1, 3
关键词: Recycle preparative HPLC ;  Repetitive enrichment ;  Low abundance compound ;  Column capture ;  Mobile phase adjusting
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-07-18
DOI: 10.1016/j.chroma.2014.05.041
卷: 1351, 页:90-95
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel recycle preparative HPLC system was developed to repeatedly enrich and purify low-abundance compounds from complex samples. The recycle separation involves injecting a large volume of the sample through a trap column, releasing the absorbed sample for preparative HPLC separation, and then capturing the target component in the same trap column for the next separation. After several cycles, the final purified target compound absorbed in the trap column is eluted with a small volume of solvent. The capture and recycle procedure was realized by adjusting the strength of the mobile phase using an auxiliary pump and switching the two valves connecting the trap and preparative columns. Compared with standard HPLC or existing recycle chromatography, this system not only reduces the solvent side-effects and sample volume overloading during injection but also avoids peak-broadening and sensitivity loss during the recycle runs. Moreover, the final purified products can be easily concentrated. With the aid of this system, a 10% polyphenol in extracts from Saussurea involucrata cultured cells reached >95% purity with more than 95% recovery after three recycle purifications, and a 1% unstable epimer impurity in the raw material of the drug silybin reached >95% purity with 75% recovery after a six-cycle separation. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: RECYCLE CHROMATOGRAPHY ;  SAUSSUREA-INVOLUCRATA ;  PHASE ;  INJECTION ;  SOLVENTS ;  DESIGN ;  SYSTEM
语种: 英语
WOS记录号: WOS:000337990400011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145502
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dept Biosci & Biotechnol, Dalian 116024, Peoples R China
3.Chinese Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

Recommended Citation:
Zou, Xiaowei,Gao, Mingzhe,Liu, Dan,et al. A novel preparative liquid chromatograph for repetitive enrichment and purification of low-abundance compounds[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1351:90-95.
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