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题名: Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions
作者: Meng, Yingying1, 2;  Yao, Changhong1, 3;  Xue, Song1;  Yang, Haibo2
关键词: FT-IR ;  Microalgae ;  Cell composition ;  Strain screening ;  Physiological status
刊名: BIORESOURCE TECHNOLOGY
发表日期: 2014
DOI: 10.1016/j.biortech.2013.10.064
卷: 151, 页:347-354
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Agricultural Engineering ;  Biotechnology & Applied Microbiology ;  Energy & Fuels
研究领域[WOS]: Agriculture ;  Biotechnology & Applied Microbiology ;  Energy & Fuels
英文摘要: Fourier transform infrared spectroscopy (FT-IR) was applied in algal strain screening and monitoring cell composition dynamics in a marine microalga Isochrysis zhangjiangensis during algal cultivation. The content of lipid, carbohydrate and protein of samples determined by traditional methods had validated the accuracy of FT-IR method. For algal screening, the band absorption ratios of lipid/amide I and carbo/amide I from FT-IR measurements allowed for the selection of Isochrysis sp. and Tetraselmis subcordiformis as the most potential lipid and carbohydrate producers, respectively. The cell composition dynamics oft zhangjiangensis measured by FT-IR revealed the diversion of carbon allocation from protein to carbohydrate and neutral lipid when nitrogen-replete cells were subjected to nitrogen limitation. The carbo/amide I band absorption ratio had also been demonstrated to depict physiological status under nutrient stress in T. subcordiformis. FT-IR serves as a tool for the simultaneous measurement of lipid, carbohydrate, and protein content in cell. (C) 2013 Elsevier Ltd. All rights reserved.
关键词[WOS]: BIOMASS COMPOSITION ;  CHLAMYDOMONAS-REINHARDTII ;  CARBON ALLOCATION ;  ALGAL BIOMASS ;  SPECTRA ;  MICROSPECTROSCOPY ;  PHYTOPLANKTON ;  ACCUMULATION ;  PROTEIN ;  STARCH
语种: 英语
WOS记录号: WOS:000330085800048
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145512
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Meng, Yingying,Yao, Changhong,Xue, Song,et al. Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions[J]. BIORESOURCE TECHNOLOGY,2014,151:347-354.
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