DICP OpenIR
Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions
Meng, Yingying1,2; Yao, Changhong1,3; Xue, Song1; Yang, Haibo2
KeywordFt-ir Microalgae Cell Composition Strain Screening Physiological Status
Source PublicationBIORESOURCE TECHNOLOGY
2014
DOI10.1016/j.biortech.2013.10.064
Volume151Pages:347-354
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS KeywordBIOMASS COMPOSITION ; CHLAMYDOMONAS-REINHARDTII ; CARBON ALLOCATION ; ALGAL BIOMASS ; SPECTRA ; MICROSPECTROSCOPY ; PHYTOPLANKTON ; ACCUMULATION ; PROTEIN ; STARCH
AbstractFourier transform infrared spectroscopy (FT-IR) was applied in algal strain screening and monitoring cell composition dynamics in a marine microalga Isochrysis zhangjiangensis during algal cultivation. The content of lipid, carbohydrate and protein of samples determined by traditional methods had validated the accuracy of FT-IR method. For algal screening, the band absorption ratios of lipid/amide I and carbo/amide I from FT-IR measurements allowed for the selection of Isochrysis sp. and Tetraselmis subcordiformis as the most potential lipid and carbohydrate producers, respectively. The cell composition dynamics oft zhangjiangensis measured by FT-IR revealed the diversion of carbon allocation from protein to carbohydrate and neutral lipid when nitrogen-replete cells were subjected to nitrogen limitation. The carbo/amide I band absorption ratio had also been demonstrated to depict physiological status under nutrient stress in T. subcordiformis. FT-IR serves as a tool for the simultaneous measurement of lipid, carbohydrate, and protein content in cell. (C) 2013 Elsevier Ltd. All rights reserved.
Language英语
WOS IDWOS:000330085800048
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145512
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Meng, Yingying,Yao, Changhong,Xue, Song,et al. Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions[J]. BIORESOURCE TECHNOLOGY,2014,151:347-354.
APA Meng, Yingying,Yao, Changhong,Xue, Song,&Yang, Haibo.(2014).Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions.BIORESOURCE TECHNOLOGY,151,347-354.
MLA Meng, Yingying,et al."Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions".BIORESOURCE TECHNOLOGY 151(2014):347-354.
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