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题名: Surface Protein Imprinted Core-Shell Particles for High Selective Lysozyme Recognition Prepared by Reversible Addition-Fragmentation Chain Transfer Strategy
作者: Li, Qinran1, 2;  Yang, Kaiguang1;  Liang, Yu1;  Jiang, Bo1, 2;  Liu, Jianxi1, 2;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Yukui1
关键词: molecularly imprinting ;  RAFT ;  protein recognition ;  core-shell particles ;  lysozyme
刊名: ACS APPLIED MATERIALS & INTERFACES
发表日期: 2014-12-24
DOI: 10.1021/am5072783
卷: 6, 期:24, 页:21954-21960
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Nanoscience & Nanotechnology ;  Materials Science, Multidisciplinary
研究领域[WOS]: Science & Technology - Other Topics ;  Materials Science
英文摘要: A novel kind of lysozyme (Lys) surface imprinted core-shell particles was synthesized by reversible addition-fragmentation chain transfer (RAFT) strategy. With controllable polymer shell chain length, such particles showed obviously improved selectivity for protein recognition. After the RAFT initial agent and template protein was absorbed on silica particles, the prepolymerization solution, with methacrylic acid and 2-hydroxyethyl methacrylate as the monomers, and N,N'-methylenebis(acrylamide) as the cross-linker, was mixed with the silica particles, and the polymerization was performed at 40 degrees C in aqueous phase through the oxidation-reduction initiation. Ater polymerization, with the template protein removal and destroying dithioester groups with hexylamine, the surface Lyz imprinted particles were obtained with controllable polymer chain length. The binding capacity of the Lys imprinted particles could reach 5.6 mg protein/g material, with the imprinting factor (IF) as 3.7, whereas the IF of the control material prepared without RAFT strategy was only 1.6. The absorption equilibrium could be achieved within 60 min. Moreover, Lys could be selectively recognized by the imprinted particles from both a four-proteins mixture and egg white sample. All these results demonstrated that these particles prepared by RAFT strategy are promising to achieve the protein recognition with high selectivity.
关键词[WOS]: TRANSFER POLYMERIZATION ;  RAFT POLYMERIZATION ;  RADICAL POLYMERIZATION ;  MOLECULAR RECOGNITION ;  POLYMERS ;  WATER ;  NANOPARTICLES ;  SILICA ;  BRUSHES ;  SERUM
语种: 英语
WOS记录号: WOS:000347139400026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145522
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Li, Qinran,Yang, Kaiguang,Liang, Yu,et al. Surface Protein Imprinted Core-Shell Particles for High Selective Lysozyme Recognition Prepared by Reversible Addition-Fragmentation Chain Transfer Strategy[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(24):21954-21960.
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