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题名: A combination of distillation-precipitation polymerization and click chemistry: fabrication of boronic acid functionalized Fe3O4 hybrid composites for enrichment of glycoproteins
作者: Zhang, Xihao1, 2;  He, Xiwen1, 2;  Chen, Langxing1, 2;  Zhang, Yukui1, 2, 3
刊名: JOURNAL OF MATERIALS CHEMISTRY B
发表日期: 2014
DOI: 10.1039/c4tb00379a
卷: 2, 期:21, 页:3254-3262
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Materials Science, Biomaterials
研究领域[WOS]: Materials Science
英文摘要: Biomedical sciences, and in particular biomarker research, demand efficient glycoprotein enrichment platforms. In this paper, a facile and efficient approach combining distillation-precipitation polymerization (DPP) and click chemistry was developed to synthesize boronic acid ligand-modified magnetic nanoparticles for the enrichment of glycoproteins. Due to the relatively large amount of benzyl chloride groups introduced by DPP on the magnetic core, which easily can be transferred into azide groups, the alkyne-phenylboronic acid ligands were immobilized onto the surface of Fe3O4 with high efficiency via the Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) 'click' reaction. The morphology, structure and composition of the resulting core-shell Fe3O4@poly(4-vinylbenylchloride)@amidophenylboronic acid (Fe3O4@pVBC@APBA) nanocomposites were characterized by transmission electron microscopy, X-ray powder diffraction, vibrating sample magnetometry, Fourier transform infrared spectroscopy, thermogravimetric analysis and X-ray photoelectron spectrometry. The Fe3O4@pVBC@APBA microspheres held a similar to 50 nm polymeric shell, and exhibited high magnetic response to an external magnetic field. The binding results demonstrated that Fe3O4@pVBC@APBA possessed high adsorption capacity and remarkable selectivity to glycoproteins. Moreover, the glycoproteins in the egg white sample could be enriched under physiological conditions (pH 7.4) as well, due to the lower pK(a) value of the alkyne-phenylboronic acid ligand. The high stability and selectivity of Fe3O4@pVBC@APBA for the glycoproteins were retained over several separation cycles. This boronate affinity material has potential applications in biomedical and biotechnological fields including drug delivery and biosensing.
关键词[WOS]: AFFINITY MONOLITHIC COLUMN ;  IRON-OXIDE NANOPARTICLES ;  N-LINKED GLYCOPROTEINS ;  MAGNETIC NANOPARTICLES ;  SELECTIVE ENRICHMENT ;  CIS-DIOL ;  BIOMEDICAL APPLICATIONS ;  EFFICIENT STRATEGY ;  NEUTRAL CONDITIONS ;  MASS-SPECTROMETRY
语种: 英语
WOS记录号: WOS:000336072400010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145524
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Coll Chem, Res Ctr Analyt Sci, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Collaborat Innovat Ctr Chem Sci & Engn, Tianjin, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, Xihao,He, Xiwen,Chen, Langxing,et al. A combination of distillation-precipitation polymerization and click chemistry: fabrication of boronic acid functionalized Fe3O4 hybrid composites for enrichment of glycoproteins[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(21):3254-3262.
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