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A combination of distillation-precipitation polymerization and click chemistry: fabrication of boronic acid functionalized Fe3O4 hybrid composites for enrichment of glycoproteins
Zhang, Xihao1,2; He, Xiwen1,2; Chen, Langxing1,2; Zhang, Yukui1,2,3
Source PublicationJOURNAL OF MATERIALS CHEMISTRY B
2014
DOI10.1039/c4tb00379a
Volume2Issue:21Pages:3254-3262
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectMaterials Science, Biomaterials
WOS Research AreaMaterials Science
WOS KeywordAFFINITY MONOLITHIC COLUMN ; IRON-OXIDE NANOPARTICLES ; N-LINKED GLYCOPROTEINS ; MAGNETIC NANOPARTICLES ; SELECTIVE ENRICHMENT ; CIS-DIOL ; BIOMEDICAL APPLICATIONS ; EFFICIENT STRATEGY ; NEUTRAL CONDITIONS ; MASS-SPECTROMETRY
AbstractBiomedical sciences, and in particular biomarker research, demand efficient glycoprotein enrichment platforms. In this paper, a facile and efficient approach combining distillation-precipitation polymerization (DPP) and click chemistry was developed to synthesize boronic acid ligand-modified magnetic nanoparticles for the enrichment of glycoproteins. Due to the relatively large amount of benzyl chloride groups introduced by DPP on the magnetic core, which easily can be transferred into azide groups, the alkyne-phenylboronic acid ligands were immobilized onto the surface of Fe3O4 with high efficiency via the Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) 'click' reaction. The morphology, structure and composition of the resulting core-shell Fe3O4@poly(4-vinylbenylchloride)@amidophenylboronic acid (Fe3O4@pVBC@APBA) nanocomposites were characterized by transmission electron microscopy, X-ray powder diffraction, vibrating sample magnetometry, Fourier transform infrared spectroscopy, thermogravimetric analysis and X-ray photoelectron spectrometry. The Fe3O4@pVBC@APBA microspheres held a similar to 50 nm polymeric shell, and exhibited high magnetic response to an external magnetic field. The binding results demonstrated that Fe3O4@pVBC@APBA possessed high adsorption capacity and remarkable selectivity to glycoproteins. Moreover, the glycoproteins in the egg white sample could be enriched under physiological conditions (pH 7.4) as well, due to the lower pK(a) value of the alkyne-phenylboronic acid ligand. The high stability and selectivity of Fe3O4@pVBC@APBA for the glycoproteins were retained over several separation cycles. This boronate affinity material has potential applications in biomedical and biotechnological fields including drug delivery and biosensing.
Language英语
WOS IDWOS:000336072400010
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145524
Collection中国科学院大连化学物理研究所
Affiliation1.Nankai Univ, Coll Chem, Res Ctr Analyt Sci, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Collaborat Innovat Ctr Chem Sci & Engn, Tianjin, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Zhang, Xihao,He, Xiwen,Chen, Langxing,et al. A combination of distillation-precipitation polymerization and click chemistry: fabrication of boronic acid functionalized Fe3O4 hybrid composites for enrichment of glycoproteins[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(21):3254-3262.
APA Zhang, Xihao,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2014).A combination of distillation-precipitation polymerization and click chemistry: fabrication of boronic acid functionalized Fe3O4 hybrid composites for enrichment of glycoproteins.JOURNAL OF MATERIALS CHEMISTRY B,2(21),3254-3262.
MLA Zhang, Xihao,et al."A combination of distillation-precipitation polymerization and click chemistry: fabrication of boronic acid functionalized Fe3O4 hybrid composites for enrichment of glycoproteins".JOURNAL OF MATERIALS CHEMISTRY B 2.21(2014):3254-3262.
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