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题名: Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium
作者: Wang, Xiaohui1, 2, 3, 5;  Zhao, Yong1;  Tan, Haidong1;  Chi, Naiyu2;  Zhang, Qingfang2;  Du, Yuguang4;  Yin, Heng1
关键词: Chitinase ;  Purification ;  Characterisation
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
发表日期: 2014-09-01
DOI: 10.1016/j.ijbiomac.2014.07.033
卷: 70, 页:455-462
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: In this study, we isolated a new psychrophilic bacterium, Pseudoalteromonas sp. DL-6 from marine sediments, which grew well on chitin-containing plates at 4 degrees C. One endo-type chitinase gene, chiA, was cloned from the genomic DNA of this bacterium and heterologously expressed in Escherichia coli BL21 (DE3). ChiA showed very high catalytic activity, even at 4 degrees C, and exhibited maximal activity on a chitinous substrate at pH 8.0 and 20 degrees C. Kinetic studies indicated that ChiA has a greater catalytic efficiency on 4-methylumbelliferyl-beta-D-N,N',N"-triacetylchitotriose[4-MU(GlcNAc)(3)] than on 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside[4-MU(GlcNAc)(2)]. Electrospray ionisation mass spectrometry (ESI-MS) analysis showed that the hydrolysis products of powdered chitin after ChiA digestion consisted of a series of chitin oligomers with different degrees of polymerisation. The ChiA mode of action was also examined using (GlcNAc)(2-6) as a substrate, and the results suggested that ChiA is a non-processive endo-type chitinase. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: SP STRAIN O-7 ;  SERRATIA-MARCESCENS ;  RECALCITRANT POLYSACCHARIDES ;  FAMILY-18 CHITINASE ;  MOLECULAR ANALYSIS ;  MORITELLA-MARINA ;  BINDING DOMAIN ;  GENE ;  PROCESSIVITY ;  DEGRADATION
语种: 英语
WOS记录号: WOS:000342478800065
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145567
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ, Sch Life Sci & Biotechnol, Dalian 116622, Peoples R China
3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Xiaohui,Zhao, Yong,Tan, Haidong,et al. Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2014,70:455-462.
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