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题名: Highly efficient N-glycoproteomic sample preparation by combining C-18 and graphitized carbon adsorbents
作者: Liu, Jing1, 2;  Wang, Fangjun1;  Zhu, Jun1, 2;  Mao, Jiawei1, 2;  Liu, Zheyi1, 2;  Cheng, Kai1, 2;  Qin, Hongqiang1;  Zou, Hanfa1
关键词: Porous graphitized carbon ;  C-18 adsorbent ;  N-glycoproteomic sample preparation ;  Proteomics ;  LC-MS/MS
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2014-05-01
DOI: 10.1007/s00216-014-7716-9
卷: 406, 期:13, 页:3103-3109
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Conventional N-glycoproteome analysis usually applies C-18 reversed-phase (RP) adsorbent for sample purification, which will lead to unavoidable sample loss due to the high hydrophilicity of N-glycopeptides. In this study, a porous graphitized carbon (PGC) absorbent was combined with a C-18 adsorbent for N-glycopeptide purification in comprehensive N-glycoproteome analysis based on the hydrophobic and polar interactions between carbon and N-glycans. It was observed that the small hydrophilic N-glycopeptides that cannot retain onto C-18 adsorbent can be captured by the graphitized carbon, while the large hydrophobic N-glycopeptides that cannot retain onto the graphitized carbon can be feasibly captured by the C-18 adsorbent. Comparing with sample purification by using C-18 adsorbent only, 28.5 % more N-glycopeptides were identified by combining both C-18 and PGC adsorbents. The C-18-PGC strategy was further applied for both sample purification and pre-fractionation of a complex protein sample from HeLa cell. After hydrophilic interaction chromatography enrichment, 1,484 unique N-glycopeptides with 1,759 unique N-glycosylation sites were finally identified.
关键词[WOS]: LIQUID-CHROMATOGRAPHY ;  GLYCANS ;  PHASE ;  OLIGOSACCHARIDES ;  GLYCOPEPTIDES ;  GLYCOSYLATION ;  PROTEOMICS ;  PEPTIDES ;  PROTEIN ;  CANCER
语种: 英语
WOS记录号: WOS:000334685400011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145575
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Liu, Jing,Wang, Fangjun,Zhu, Jun,et al. Highly efficient N-glycoproteomic sample preparation by combining C-18 and graphitized carbon adsorbents[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2014,406(13):3103-3109.
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