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题名: Efficient purification of active bufadienolides by a class separation method based on hydrophilic solid-phase extraction and reversed-phase high performance liquid chromatography
作者: Li, Xiaolong1, 2, 3;  Liu, Yanfang1;  Shen, Aijin1;  Wang, Chaoran1;  Yan, Jingyu1;  Zhao, Weijie2;  Liang, Xinmiao1
关键词: Active bufadienolides ;  Hydrophilic solid-phase extraction ;  Class separation ;  Purification ;  Toad skin
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2014-08-25
DOI: 10.1016/j.jpba.2014.04.015
卷: 97, 页:54-64
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: Traditional Chinese medicines (TCMs) have played a significant role in the process of discovering natural bioactive compounds, especially in anticancer therapeutics. However, the components of TCMs are complex mixtures with wide variation in polarity and content, which leads to inefficiency in the process of active compound discovery from TCMs. In this paper, the popular strategy of utilizing "pre-fractionated natural product libraries" has been improved by a new class separation approach to accelerate the process. As an example, the skin of Bufo bufo gargarizans Cantor, a well-known TCM, mainly contains two distinct bufadienolide classes: amino acid-conjugated bufadienolides (AACBs) and free form bufadienolides (AAUBs). We utilized hydrophilic interaction liquid chromatography solid-phase extraction (HILIC-SPE) to resolve the two types of bufadienolides, which co-eluted on C18 columns. By this strategy, twelve bufadienolides of the two types were purified via prep-HPLC from one active fraction, and eight of them were identified by H-1 NMR and C-13 NMR. These results indicated that the class separation method not only overcame the limited orthogonality in a 2D-RPLC x RPLC system but also accelerated the process of active compound discovery. (c) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: BUFO-BUFO-GARGARIZANS ;  CHARGED C18 COLUMN ;  DRUG CHAN-SU ;  NATURAL-PRODUCTS ;  CYTOTOXIC CONSTITUENTS ;  TOAD ;  SKIN ;  DISCOVERY ;  BIOTRANSFORMATION ;  SECRETION
语种: 英语
WOS记录号: WOS:000337780900008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145625
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Sch Pharmaceut Sci & Technol, Dalian 116024, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Li, Xiaolong,Liu, Yanfang,Shen, Aijin,et al. Efficient purification of active bufadienolides by a class separation method based on hydrophilic solid-phase extraction and reversed-phase high performance liquid chromatography[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,97:54-64.
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