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Metabolomics study of cured tobacco using liquid chromatography with mass spectrometry: Method development and its application in investigating the chemical differences of tobacco from three growing regions
Li, Lili; Zhao, Chunxia; Chang, Yuwei; Lu, Xin; Zhang, Junjie; Zhao, Yanni; Zhao, Jieyu; Xu, Guowang
关键词Chemical Fingerprint Cured Tobacco Plant Metabolomics Regional Variation Ms Ms
刊名JOURNAL OF SEPARATION SCIENCE
2014-05-01
DOI10.1002/jssc.201301138
37期:9-10页:1067-1074
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PLANT METABOLOMICS ; FUNCTIONAL GENOMICS ; QUALITY-CONTROL ; GC-MS ; LEAVES ; METABOLITES ; EXTRACTION ; STRATEGIES ; SEPARATION ; DATABASE
英文摘要Cured tobacco is an important plant material. Component studies are a big challenge for its significantly diverse chemical properties and vastly different concentrations. In this work, liquid chromatography with quadrupole time-of-flight mass spectrometry was used to perform a metabolomics study of cured tobacco owing to its efficient separation and detection of semipolar metabolites. A solvent of methanol/water (8:2, v/v) and 30 min of ultrasound time were found to be optimal to perform extraction. 95, 92, and 93% of metabolite features had within 20% of coefficient of variation for repeatability, intraday and interday precision analysis, respectively, indicating a good stability of the method developed. 113 metabolites were identified in cured tobacco based on accurate mass, retention time, and MS/MS fragments. The developed method was applied to a metabolomics study of cured tobacco from three growing regions. Forty three metabolites were found to be contributed to the classification. It is shown that the developed method can be applied to metabolomics analysis of plant materials.
语种英语
WOS记录号WOS:000335196500003
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145646
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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Li, Lili,Zhao, Chunxia,Chang, Yuwei,et al. Metabolomics study of cured tobacco using liquid chromatography with mass spectrometry: Method development and its application in investigating the chemical differences of tobacco from three growing regions[J]. JOURNAL OF SEPARATION SCIENCE,2014,37(9-10):1067-1074.
APA Li, Lili.,Zhao, Chunxia.,Chang, Yuwei.,Lu, Xin.,Zhang, Junjie.,...&Xu, Guowang.(2014).Metabolomics study of cured tobacco using liquid chromatography with mass spectrometry: Method development and its application in investigating the chemical differences of tobacco from three growing regions.JOURNAL OF SEPARATION SCIENCE,37(9-10),1067-1074.
MLA Li, Lili,et al."Metabolomics study of cured tobacco using liquid chromatography with mass spectrometry: Method development and its application in investigating the chemical differences of tobacco from three growing regions".JOURNAL OF SEPARATION SCIENCE 37.9-10(2014):1067-1074.
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