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题名: Metabolomics study of cured tobacco using liquid chromatography with mass spectrometry: Method development and its application in investigating the chemical differences of tobacco from three growing regions
作者: Li, Lili1;  Zhao, Chunxia1;  Chang, Yuwei1;  Lu, Xin1;  Zhang, Junjie1;  Zhao, Yanni1;  Zhao, Jieyu1;  Xu, Guowang1
关键词: Chemical fingerprint ;  Cured tobacco ;  Plant metabolomics ;  Regional variation ;  MS ;  MS
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2014-05-01
DOI: 10.1002/jssc.201301138
卷: 37, 期:9-10, 页:1067-1074
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Cured tobacco is an important plant material. Component studies are a big challenge for its significantly diverse chemical properties and vastly different concentrations. In this work, liquid chromatography with quadrupole time-of-flight mass spectrometry was used to perform a metabolomics study of cured tobacco owing to its efficient separation and detection of semipolar metabolites. A solvent of methanol/water (8:2, v/v) and 30 min of ultrasound time were found to be optimal to perform extraction. 95, 92, and 93% of metabolite features had within 20% of coefficient of variation for repeatability, intraday and interday precision analysis, respectively, indicating a good stability of the method developed. 113 metabolites were identified in cured tobacco based on accurate mass, retention time, and MS/MS fragments. The developed method was applied to a metabolomics study of cured tobacco from three growing regions. Forty three metabolites were found to be contributed to the classification. It is shown that the developed method can be applied to metabolomics analysis of plant materials.
关键词[WOS]: PLANT METABOLOMICS ;  FUNCTIONAL GENOMICS ;  QUALITY-CONTROL ;  GC-MS ;  LEAVES ;  METABOLITES ;  EXTRACTION ;  STRATEGIES ;  SEPARATION ;  DATABASE
语种: 英语
WOS记录号: WOS:000335196500003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145646
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Li, Lili,Zhao, Chunxia,Chang, Yuwei,et al. Metabolomics study of cured tobacco using liquid chromatography with mass spectrometry: Method development and its application in investigating the chemical differences of tobacco from three growing regions[J]. JOURNAL OF SEPARATION SCIENCE,2014,37(9-10):1067-1074.
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