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Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation
You, Xiao-Guang1,2; Tu, Rong1,2; Peng, Ming-Li3; Bai, Yu-Jie1,2; Tan, Mingqian4; Li, Han-Jian1,2; Guan, Jing5; Wen, Li-Jun6
关键词Vegf165 Aptamer (Uspio) Targeted Molecular Probe Mri Liver Cancer
刊名CONTRAST MEDIA & MOLECULAR IMAGING
2014-09-01
DOI10.1002/cmmi.1584
9期:5页:349-354
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]ENDOTHELIAL GROWTH-FACTOR ; CONTRAST AGENTS ; ANTI-VEGF ; CANCER ; ANGIOGENESIS ; NANOPARTICLES ; DESIGN
英文摘要A new method for imaging the tumor human vascular endothelial growth factor 165 (VEGF 165) is presented. A magnetic resonance imaging (MRI) probe was prepared by crosslinking ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles to the aptamer for tumor vascular endothelial growth factor 165 (VEGF165-aptamer). The molecular probe was evaluated for its in vitro and in vivo activities toward VEGF165. Enzyme-linked immunosorbent assay showed that the VEGF165-aptamer-USPIO nanoparticles conjugate specifically binds to VEGF165 in vitro. A cell proliferation test showed that VEGF165-aptamer-USPIO seems to block the proliferation of human umbilical vein endothelial cells induced by free VEGF165, suggesting that VEGF165 is an effective target of this molecular probe. In xenograft mice carrying liver cancer that expresses VEGF165, T-2-weighted imaging of the tumor displayed marked negative enhancement 3h after the intravenous administration of VEGF165-aptamer-USPIO. The enhancement disappeared 6h after administration of the probe. These results suggest the targeted imaging effect of VEGF165-aptamer-USPIO probe in vivo for VEGF165-expressing tumors. This is the first report of a targeted MRI molecular probe based on USPIO and VEGF165-aptamer. Copyright (c) 2014 John Wiley & Sons, Ltd.
语种英语
WOS记录号WOS:000343878700004
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145654
专题中国科学院大连化学物理研究所
作者单位1.Hainan Med Coll, Dept Radiol, Haikou 570102, Hainan Province, Peoples R China
2.Hainan Med Coll, Inst Canc, Haikou 570102, Hainan Province, Peoples R China
3.Northwest Univ, Xian 710068, Shanxi Province, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
5.Zhunyi Med Coll Hop, Dept Radiol, Zhunyi City 564400, Guizhou Provinc, Peoples R China
6.Hainan Med Coll, Sch Pharm, Haikou 571101, Hainan Province, Peoples R China
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You, Xiao-Guang,Tu, Rong,Peng, Ming-Li,et al. Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation[J]. CONTRAST MEDIA & MOLECULAR IMAGING,2014,9(5):349-354.
APA You, Xiao-Guang.,Tu, Rong.,Peng, Ming-Li.,Bai, Yu-Jie.,Tan, Mingqian.,...&Wen, Li-Jun.(2014).Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation.CONTRAST MEDIA & MOLECULAR IMAGING,9(5),349-354.
MLA You, Xiao-Guang,et al."Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation".CONTRAST MEDIA & MOLECULAR IMAGING 9.5(2014):349-354.
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