DICP OpenIR
Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation
You, Xiao-Guang1,2; Tu, Rong1,2; Peng, Ming-Li3; Bai, Yu-Jie1,2; Tan, Mingqian4; Li, Han-Jian1,2; Guan, Jing5; Wen, Li-Jun6
KeywordVegf165 Aptamer (Uspio) Targeted Molecular Probe Mri Liver Cancer
Source PublicationCONTRAST MEDIA & MOLECULAR IMAGING
2014-09-01
DOI10.1002/cmmi.1584
Volume9Issue:5Pages:349-354
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectRadiology, Nuclear Medicine & Medical Imaging
WOS Research AreaRadiology, Nuclear Medicine & Medical Imaging
WOS KeywordENDOTHELIAL GROWTH-FACTOR ; CONTRAST AGENTS ; ANTI-VEGF ; CANCER ; ANGIOGENESIS ; NANOPARTICLES ; DESIGN
AbstractA new method for imaging the tumor human vascular endothelial growth factor 165 (VEGF 165) is presented. A magnetic resonance imaging (MRI) probe was prepared by crosslinking ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles to the aptamer for tumor vascular endothelial growth factor 165 (VEGF165-aptamer). The molecular probe was evaluated for its in vitro and in vivo activities toward VEGF165. Enzyme-linked immunosorbent assay showed that the VEGF165-aptamer-USPIO nanoparticles conjugate specifically binds to VEGF165 in vitro. A cell proliferation test showed that VEGF165-aptamer-USPIO seems to block the proliferation of human umbilical vein endothelial cells induced by free VEGF165, suggesting that VEGF165 is an effective target of this molecular probe. In xenograft mice carrying liver cancer that expresses VEGF165, T-2-weighted imaging of the tumor displayed marked negative enhancement 3h after the intravenous administration of VEGF165-aptamer-USPIO. The enhancement disappeared 6h after administration of the probe. These results suggest the targeted imaging effect of VEGF165-aptamer-USPIO probe in vivo for VEGF165-expressing tumors. This is the first report of a targeted MRI molecular probe based on USPIO and VEGF165-aptamer. Copyright (c) 2014 John Wiley & Sons, Ltd.
Language英语
WOS IDWOS:000343878700004
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145654
Collection中国科学院大连化学物理研究所
Affiliation1.Hainan Med Coll, Dept Radiol, Haikou 570102, Hainan Province, Peoples R China
2.Hainan Med Coll, Inst Canc, Haikou 570102, Hainan Province, Peoples R China
3.Northwest Univ, Xian 710068, Shanxi Province, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
5.Zhunyi Med Coll Hop, Dept Radiol, Zhunyi City 564400, Guizhou Provinc, Peoples R China
6.Hainan Med Coll, Sch Pharm, Haikou 571101, Hainan Province, Peoples R China
Recommended Citation
GB/T 7714
You, Xiao-Guang,Tu, Rong,Peng, Ming-Li,et al. Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation[J]. CONTRAST MEDIA & MOLECULAR IMAGING,2014,9(5):349-354.
APA You, Xiao-Guang.,Tu, Rong.,Peng, Ming-Li.,Bai, Yu-Jie.,Tan, Mingqian.,...&Wen, Li-Jun.(2014).Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation.CONTRAST MEDIA & MOLECULAR IMAGING,9(5),349-354.
MLA You, Xiao-Guang,et al."Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation".CONTRAST MEDIA & MOLECULAR IMAGING 9.5(2014):349-354.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[You, Xiao-Guang]'s Articles
[Tu, Rong]'s Articles
[Peng, Ming-Li]'s Articles
Baidu academic
Similar articles in Baidu academic
[You, Xiao-Guang]'s Articles
[Tu, Rong]'s Articles
[Peng, Ming-Li]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[You, Xiao-Guang]'s Articles
[Tu, Rong]'s Articles
[Peng, Ming-Li]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.