DICP OpenIR
Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification
Hu, Lianghai1,2; Boos, Karl-Siegfried3; Ye, Mingliang2; Zou, Hanfa2
KeywordRestrict-access Materials Sample Extraction Proteomics Serum Peptides Two-dimensional
Source PublicationTALANTA
2014-09-01
DOI10.1016/j.talanta.2014.04.011
Volume127Pages:191-195
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMULTIDIMENSIONAL LIQUID-CHROMATOGRAPHY ; RP-RP-HPLC ; SAMPLE PREPARATION ; SELECTIVE EXTRACTION ; MASS-SPECTROMETRY ; BIOLOGICAL-FLUIDS ; LC-MS/MS ; PEPTIDOMICS ; SEPARATION ; NANOPARTICLES
AbstractThe selective extraction of endogenous serum peptides has been a challenge due to the high abundant proteins present in serum. Here a simple on-line extraction of peptides from human serum using strong cation-exchange diol silica restricted-access materials (SCX-RAM) coupled with two-dimensional RP-RP liquid chromatography mass spectrometry was developed. The operation of the on-line extraction system is simple to use and does not need complex equipments. The two-dimensional RP-RP was proved to be orthogonal and efficient to separate peptides extracted from human serum. (C) 2014 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000338620300026
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145700
Collection中国科学院大连化学物理研究所
Affiliation1.Jilin Univ, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Hosp Grosshadem, Inst Clin Chem, Lab BioSeparat, D-81366 Munich, Germany
Recommended Citation
GB/T 7714
Hu, Lianghai,Boos, Karl-Siegfried,Ye, Mingliang,et al. Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification[J]. TALANTA,2014,127:191-195.
APA Hu, Lianghai,Boos, Karl-Siegfried,Ye, Mingliang,&Zou, Hanfa.(2014).Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification.TALANTA,127,191-195.
MLA Hu, Lianghai,et al."Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification".TALANTA 127(2014):191-195.
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