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题名: Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification
作者: Hu, Lianghai1, 2;  Boos, Karl-Siegfried3;  Ye, Mingliang2;  Zou, Hanfa2
关键词: Restrict-access materials ;  Sample extraction ;  Proteomics ;  Serum peptides ;  Two-dimensional
刊名: TALANTA
发表日期: 2014-09-01
DOI: 10.1016/j.talanta.2014.04.011
卷: 127, 页:191-195
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The selective extraction of endogenous serum peptides has been a challenge due to the high abundant proteins present in serum. Here a simple on-line extraction of peptides from human serum using strong cation-exchange diol silica restricted-access materials (SCX-RAM) coupled with two-dimensional RP-RP liquid chromatography mass spectrometry was developed. The operation of the on-line extraction system is simple to use and does not need complex equipments. The two-dimensional RP-RP was proved to be orthogonal and efficient to separate peptides extracted from human serum. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: MULTIDIMENSIONAL LIQUID-CHROMATOGRAPHY ;  RP-RP-HPLC ;  SAMPLE PREPARATION ;  SELECTIVE EXTRACTION ;  MASS-SPECTROMETRY ;  BIOLOGICAL-FLUIDS ;  LC-MS/MS ;  PEPTIDOMICS ;  SEPARATION ;  NANOPARTICLES
语种: 英语
WOS记录号: WOS:000338620300026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145700
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Jilin Univ, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Hosp Grosshadem, Inst Clin Chem, Lab BioSeparat, D-81366 Munich, Germany

Recommended Citation:
Hu, Lianghai,Boos, Karl-Siegfried,Ye, Mingliang,et al. Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification[J]. TALANTA,2014,127:191-195.
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