DICP OpenIR
Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification
Hu, Lianghai1,2; Boos, Karl-Siegfried3; Ye, Mingliang2; Zou, Hanfa2
关键词Restrict-access Materials Sample Extraction Proteomics Serum Peptides Two-dimensional
刊名TALANTA
2014-09-01
DOI10.1016/j.talanta.2014.04.011
127页:191-195
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MULTIDIMENSIONAL LIQUID-CHROMATOGRAPHY ; RP-RP-HPLC ; SAMPLE PREPARATION ; SELECTIVE EXTRACTION ; MASS-SPECTROMETRY ; BIOLOGICAL-FLUIDS ; LC-MS/MS ; PEPTIDOMICS ; SEPARATION ; NANOPARTICLES
英文摘要The selective extraction of endogenous serum peptides has been a challenge due to the high abundant proteins present in serum. Here a simple on-line extraction of peptides from human serum using strong cation-exchange diol silica restricted-access materials (SCX-RAM) coupled with two-dimensional RP-RP liquid chromatography mass spectrometry was developed. The operation of the on-line extraction system is simple to use and does not need complex equipments. The two-dimensional RP-RP was proved to be orthogonal and efficient to separate peptides extracted from human serum. (C) 2014 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000338620300026
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145700
专题中国科学院大连化学物理研究所
作者单位1.Jilin Univ, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Hosp Grosshadem, Inst Clin Chem, Lab BioSeparat, D-81366 Munich, Germany
推荐引用方式
GB/T 7714
Hu, Lianghai,Boos, Karl-Siegfried,Ye, Mingliang,et al. Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification[J]. TALANTA,2014,127:191-195.
APA Hu, Lianghai,Boos, Karl-Siegfried,Ye, Mingliang,&Zou, Hanfa.(2014).Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification.TALANTA,127,191-195.
MLA Hu, Lianghai,et al."Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification".TALANTA 127(2014):191-195.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Hu, Lianghai]的文章
[Boos, Karl-Siegfried]的文章
[Ye, Mingliang]的文章
百度学术
百度学术中相似的文章
[Hu, Lianghai]的文章
[Boos, Karl-Siegfried]的文章
[Ye, Mingliang]的文章
必应学术
必应学术中相似的文章
[Hu, Lianghai]的文章
[Boos, Karl-Siegfried]的文章
[Ye, Mingliang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。