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Fully Deacetylated Chitooligosaccharides Act as Efficient Glycoside Hydrolase Family 18 Chitinase Inhibitors.
Chen, Lei1; Zhou, Yong2; Qu, Mingbo1; Zhao, Yong3; Yang, Qing1
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2014-06-20
DOI10.1074/jbc.M114.564534
289期:25页:17932-17940
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]SERRATIA-MARCESCENS 2170 ; GLIOCLADIUM SP FTD-0668 ; BACTERIAL CHITINASES ; ALLOSAMIDIN ; PRODUCT ; COMPLEX ; MECHANISM ; ARGIFIN ; BINDING ; CLONING
英文摘要Small molecule inhibitors against chitinases have potential applications as pesticides, fungicides, and antiasthmatics. Here, we report that a series of fully deacetylated chitooligosaccharides (GlcN)(2-7) can act as inhibitors against the insect chitinase OfChtI, the human chitinase HsCht, and the bacterial chitinases SmChiA and SmChiB with IC50 values at micromolar to millimolar levels. The injection of mixed (GlcN)(2-7) into the fifth instar larvae of the insect Ostrinia furnacalis resulted in 85% of the larvae being arrested at the larval stage and death after 10 days, also suggesting that (GlcN)(2-7) might inhibit OfChtI in vivo. Crystal structures of the catalytic domain of OfChtI (OfChtI-CAD) complexed with (GlcN)(5,6) were obtained at resolutions of 2.0 angstrom. These structures, together with mutagenesis and thermodynamic analysis, suggested that the inhibition was strongly related to the interaction between the -1 GlcN residue of the inhibitor and the catalytic Glu(148) of the enzyme. Structure-based comparison showed that the fully deacetylated chitooligosaccharides mimic the substrate chitooligosaccharides by binding to the active cleft. This work first reports the inhibitory activity and proposed inhibitory mechanism of fully deacetylated chitooligosaccharides. Because the fully deacetylated chitooligosaccharides can be easily derived from chitin, one of the most abundant materials in nature, this work also provides a platform for developing eco-friendly inhibitors against chitinases.
语种英语
WOS记录号WOS:000338018100053
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145732
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Liaoning, Peoples R China
2.Dalian Univ Technol, Sch Software, Dalian 116620, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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Chen, Lei,Zhou, Yong,Qu, Mingbo,et al. Fully Deacetylated Chitooligosaccharides Act as Efficient Glycoside Hydrolase Family 18 Chitinase Inhibitors.[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2014,289(25):17932-17940.
APA Chen, Lei,Zhou, Yong,Qu, Mingbo,Zhao, Yong,&Yang, Qing.(2014).Fully Deacetylated Chitooligosaccharides Act as Efficient Glycoside Hydrolase Family 18 Chitinase Inhibitors..JOURNAL OF BIOLOGICAL CHEMISTRY,289(25),17932-17940.
MLA Chen, Lei,et al."Fully Deacetylated Chitooligosaccharides Act as Efficient Glycoside Hydrolase Family 18 Chitinase Inhibitors.".JOURNAL OF BIOLOGICAL CHEMISTRY 289.25(2014):17932-17940.
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