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题名: Selective Extraction of Low Molecular Weight Proteins by Mesoporous Silica Particles with Phenyl Modified Internal and Alkyl-diol Modified External Surfaces
作者: Qi Yan-Xia1, 2;  Zhao Qian-Cheng1;  Li Zhi-Bo1;  Li Wei1;  Wang Qiu-Kuan1;  Guan Ya-Feng2
关键词: Mesoporous silica ;  Internal and external surfaces modification ;  Selective extraction ;  Low molecular weight protein ;  Biological sample preparation
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2014-08-01
DOI: 10.1016/S1872-2040(14)60756-8
卷: 42, 期:8, 页:1083-1087
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Selective extraction of low molecular weight (LMW) proteins and peptides from complex biological samples plays an important role in the discovery of useful biomarkers and signaling molecules. It was demonstrated that the unique pore structure of mesoporous material made it efficient to enrich LMW proteins and peptides from complex matrixes. In this study, a mesoporous material with modified external (alkyl-diol group) and internal (phenyl group) surfaces (alkyl-diol@phenyl-SiO2) was prepared by co-condensation and post-grafting, and its characteristic was evaluated by Fourier transform-infrared spectra (FT-IR) and mass spectrometry (MS). The LMW proteins and peptides enriched by the alkyl-diol@phenyl-SiO2 mesoporous material could be easily eluted by organic solvents, which was compatible with the following detection by MS. This new mesoporous material exhibited good selectivity for the extraction of LMW proteins and peptides (less than 10 kDa) from complex biological samples.
关键词[WOS]: LIQUID-CHROMATOGRAPHY ;  SEPARATION ;  FUNCTIONALIZATION ;  ENRICHMENT ;  PEPTIDES ;  COLUMN ;  SERUM
语种: 英语
WOS记录号: WOS:000342387200003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145742
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Ocean Univ, Coll Food Sci & Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Qi Yan-Xia,Zhao Qian-Cheng,Li Zhi-Bo,et al. Selective Extraction of Low Molecular Weight Proteins by Mesoporous Silica Particles with Phenyl Modified Internal and Alkyl-diol Modified External Surfaces[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2014,42(8):1083-1087.
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