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Novel Gemini cationic lipids with carbamate groups for gene delivery
Zhao, Yi-Nan1,2; Qureshi, Farooq3; Zhang, Shu-Biao2; Cui, Shao-Hui2; Wang, Bing2; Chen, Hui-Ying2; Lv, Hong-Tao4; Zhang, Shu-Fen1; Huang, Leaf5
刊名JOURNAL OF MATERIALS CHEMISTRY B
2014
DOI10.1039/c3tb21506g
2期:19页:2920-2928
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology
类目[WOS]Materials Science, Biomaterials
研究领域[WOS]Materials Science
关键词[WOS]TRANSFECTION EFFICIENCY ; NONVIRAL VECTORS ; IN-VITRO ; DNA ; NANOPARTICLES ; DESIGN ; AMPHIPHILES ; SURFACTANTS ; POLYMERS ; CARRIERS
英文摘要To obtain efficient non-viral vectors, a series of Gemini cationic lipids with carbamate linkers between headgroups and hydrophobic tails were synthesized. They have the hydrocarbon chains of 12, 14, 16 and 18 carbon atoms as tails, designated as G12, G14, G16 and G18, respectively. These Gemini cationic lipids were prepared into cationic liposomes for the study of the physicochemical properties and gene delivery. The DNA-bonding ability of these Gemini cationic liposomes was much better than their mono-head counterparts (designated as M12, M14, M16 and M18, respectively). In the same series of liposomes, the bonding ability declined with an increase in the tail length. They were tested for their gene-transferring capabilities in Hep-2 and A549 cells. They showed a higher transfection efficiency than their mono-head counterparts and were comparable or superior in transfection efficiency and cytotoxicity to the commercial liposomes, DOTAP and Lipofectamine 2000. Our results convincingly demonstrate that the gene-transferring capabilities of these cationic lipids depended on the hydrocarbon chain length. Gene transfection efficiency was maximal at a chain length of 14, as G14 can silence about 80% of luciferase in A549 cells. Cell uptake results indicate that Gemini lipid delivery systems could be internalised by cells very efficiently. Thus, the Gemini cationic lipids could be used as synthetic non-viral gene delivery carriers for further studies.
语种英语
WOS记录号WOS:000335225100013
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145760
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116021, Liaoning, Peoples R China
2.Dalian Nationalities Univ, SEAC ME Key Lab Biotechnol & Bioresources Utiliza, Dalian 116600, Liaoning, Peoples R China
3.Pharmaceut & Analyt R&D, Roche, NJ 07110 USA
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Liaoning, Peoples R China
5.Univ N Carolina, Eshelman Sch Pharm, Div Mol Pharmaceut, Chapel Hill, NC 27515 USA
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GB/T 7714
Zhao, Yi-Nan,Qureshi, Farooq,Zhang, Shu-Biao,et al. Novel Gemini cationic lipids with carbamate groups for gene delivery[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(19):2920-2928.
APA Zhao, Yi-Nan.,Qureshi, Farooq.,Zhang, Shu-Biao.,Cui, Shao-Hui.,Wang, Bing.,...&Huang, Leaf.(2014).Novel Gemini cationic lipids with carbamate groups for gene delivery.JOURNAL OF MATERIALS CHEMISTRY B,2(19),2920-2928.
MLA Zhao, Yi-Nan,et al."Novel Gemini cationic lipids with carbamate groups for gene delivery".JOURNAL OF MATERIALS CHEMISTRY B 2.19(2014):2920-2928.
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