中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Novel Gemini cationic lipids with carbamate groups for gene delivery
作者: Zhao, Yi-Nan1, 2;  Qureshi, Farooq3;  Zhang, Shu-Biao2;  Cui, Shao-Hui2;  Wang, Bing2;  Chen, Hui-Ying2;  Lv, Hong-Tao4;  Zhang, Shu-Fen1;  Huang, Leaf5
刊名: JOURNAL OF MATERIALS CHEMISTRY B
发表日期: 2014
DOI: 10.1039/c3tb21506g
卷: 2, 期:19, 页:2920-2928
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Materials Science, Biomaterials
研究领域[WOS]: Materials Science
英文摘要: To obtain efficient non-viral vectors, a series of Gemini cationic lipids with carbamate linkers between headgroups and hydrophobic tails were synthesized. They have the hydrocarbon chains of 12, 14, 16 and 18 carbon atoms as tails, designated as G12, G14, G16 and G18, respectively. These Gemini cationic lipids were prepared into cationic liposomes for the study of the physicochemical properties and gene delivery. The DNA-bonding ability of these Gemini cationic liposomes was much better than their mono-head counterparts (designated as M12, M14, M16 and M18, respectively). In the same series of liposomes, the bonding ability declined with an increase in the tail length. They were tested for their gene-transferring capabilities in Hep-2 and A549 cells. They showed a higher transfection efficiency than their mono-head counterparts and were comparable or superior in transfection efficiency and cytotoxicity to the commercial liposomes, DOTAP and Lipofectamine 2000. Our results convincingly demonstrate that the gene-transferring capabilities of these cationic lipids depended on the hydrocarbon chain length. Gene transfection efficiency was maximal at a chain length of 14, as G14 can silence about 80% of luciferase in A549 cells. Cell uptake results indicate that Gemini lipid delivery systems could be internalised by cells very efficiently. Thus, the Gemini cationic lipids could be used as synthetic non-viral gene delivery carriers for further studies.
关键词[WOS]: TRANSFECTION EFFICIENCY ;  NONVIRAL VECTORS ;  IN-VITRO ;  DNA ;  NANOPARTICLES ;  DESIGN ;  AMPHIPHILES ;  SURFACTANTS ;  POLYMERS ;  CARRIERS
语种: 英语
WOS记录号: WOS:000335225100013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145760
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116021, Liaoning, Peoples R China
2.Dalian Nationalities Univ, SEAC ME Key Lab Biotechnol & Bioresources Utiliza, Dalian 116600, Liaoning, Peoples R China
3.Pharmaceut & Analyt R&D, Roche, NJ 07110 USA
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Liaoning, Peoples R China
5.Univ N Carolina, Eshelman Sch Pharm, Div Mol Pharmaceut, Chapel Hill, NC 27515 USA

Recommended Citation:
Zhao, Yi-Nan,Qureshi, Farooq,Zhang, Shu-Biao,et al. Novel Gemini cationic lipids with carbamate groups for gene delivery[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(19):2920-2928.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhao, Yi-Nan]'s Articles
 [Qureshi, Farooq]'s Articles
 [Zhang, Shu-Biao]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhao, Yi-Nan]‘s Articles
 [Qureshi, Farooq]‘s Articles
 [Zhang, Shu-Biao]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace