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OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC
Dang, Jun1,2; Chen, Chen2; Shao, Yun2; Mei, Lijuan2; Zhang, Huaigang2; Zhong, Zhengsheng3; Wang, Qilan2; Tao, Yanduo2
KeywordPeak Area Extraction Process Gentiana Straminea Maxim. Gentiopicroside Response Surface Methodology Hplc
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
2014-08-27
DOI10.1080/10826076.2013.825853
Volume37Issue:14Pages:1940-1952
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordASSISTED EXTRACTION ; SECOIRIDOIDS ; MACROPHYLLA
AbstractExtraction time, ratio of methanol aqueous solution to material weight, and methanol concentration for three factor and three level response surface methodology (RSM) was used to study the optimum extraction technology of gentiopicroside from Gentiana Straminea Maxim. The analysis was performed on a high performance liquid chromatography (HPLC) system with gentiopicroside peak areas as response values. A ternary quadratic polynomial model with good correlation (R-2=0.9786) was constructed and employed to guide the gentiopicroside extraction process. The optimum extraction conditions were an optimized extract time of 79.4min, a ratio of methanol aqueous solution to material weight of 23.7mL/g, methanol concentration of 92.6%, and three times extraction. Under the optimization extraction technology conditions, the predictive value of gentiopicroside peak area of the sample solution was 2120.6, and only 2.03% differ from the mean measured value 2164.6, and the optimized mean extraction yield of gentiopicroside was 78.3mg center dot g(-1). Through solvent distribution method crystallization of the extractive from response surface experiments, the white crystalline compound gentiopicroside was prepared and its purity was greater than 95%.
Language英语
WOS IDWOS:000334067600003
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145761
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Dang, Jun,Chen, Chen,Shao, Yun,et al. OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(14):1940-1952.
APA Dang, Jun.,Chen, Chen.,Shao, Yun.,Mei, Lijuan.,Zhang, Huaigang.,...&Tao, Yanduo.(2014).OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,37(14),1940-1952.
MLA Dang, Jun,et al."OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 37.14(2014):1940-1952.
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