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OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC
Dang, Jun1,2; Chen, Chen2; Shao, Yun2; Mei, Lijuan2; Zhang, Huaigang2; Zhong, Zhengsheng3; Wang, Qilan2; Tao, Yanduo2
关键词Peak Area Extraction Process Gentiana Straminea Maxim. Gentiopicroside Response Surface Methodology Hplc
刊名JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
2014-08-27
DOI10.1080/10826076.2013.825853
37期:14页:1940-1952
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]ASSISTED EXTRACTION ; SECOIRIDOIDS ; MACROPHYLLA
英文摘要Extraction time, ratio of methanol aqueous solution to material weight, and methanol concentration for three factor and three level response surface methodology (RSM) was used to study the optimum extraction technology of gentiopicroside from Gentiana Straminea Maxim. The analysis was performed on a high performance liquid chromatography (HPLC) system with gentiopicroside peak areas as response values. A ternary quadratic polynomial model with good correlation (R-2=0.9786) was constructed and employed to guide the gentiopicroside extraction process. The optimum extraction conditions were an optimized extract time of 79.4min, a ratio of methanol aqueous solution to material weight of 23.7mL/g, methanol concentration of 92.6%, and three times extraction. Under the optimization extraction technology conditions, the predictive value of gentiopicroside peak area of the sample solution was 2120.6, and only 2.03% differ from the mean measured value 2164.6, and the optimized mean extraction yield of gentiopicroside was 78.3mg center dot g(-1). Through solvent distribution method crystallization of the extractive from response surface experiments, the white crystalline compound gentiopicroside was prepared and its purity was greater than 95%.
语种英语
WOS记录号WOS:000334067600003
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145761
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
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GB/T 7714
Dang, Jun,Chen, Chen,Shao, Yun,et al. OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(14):1940-1952.
APA Dang, Jun.,Chen, Chen.,Shao, Yun.,Mei, Lijuan.,Zhang, Huaigang.,...&Tao, Yanduo.(2014).OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,37(14),1940-1952.
MLA Dang, Jun,et al."OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 37.14(2014):1940-1952.
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