DICP OpenIR
Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs
Hou, Shuhua1,2,3; Ji, Debin1,2; Liu, Wujun1,2; Wang, Lei1,2; Zhao, Zongbao (Kent)1,2
KeywordCofactor Malic Enzyme Nicotinamide Adenine Dinucleotide Orthogonal Redox System Enzyme Kinetics
Source PublicationBIOORGANIC & MEDICINAL CHEMISTRY LETTERS
2014-03-01
DOI10.1016/j.bmcl.2014.01.047
Volume24Issue:5Pages:1307-1309
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectChemistry, Medicinal ; Chemistry, Organic
WOS Research AreaPharmacology & Pharmacy ; Chemistry
WOS KeywordBIOORTHOGONAL CHEMISTRY ; SMALL MOLECULES ; NAD ANALOGS ; DISCRIMINATION ; DEHYDROGENASE ; SPECIFICITY ; SYSTEMS ; LIFE
AbstractAn activity screening between 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide (NAD) analogs and malic enzyme (ME) mutants identified some mutants capable of taking NAD analogs as the cofactor. One particular pair, ME-L310K/L404S and the analog B-8 had good catalytic efficiency and cofactor specificity. The new system gained about 1200-fold cofactor specificity shift from NAD toward B-8 in terms of oxidative decarboxylation of L-malate. Our results provided insightful information for the development of orthogonal redox system that is of particular important to precisely control engineered metabolic pathways. (C) 2014 Elsevier Ltd. All rights reserved.
Language英语
WOS IDWOS:000331705200012
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145771
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Div Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, State Key Lab Catalysis, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Bohai Univ, Dept Chem, Liaoning Key Lab Synth & Applicat Funct Cpds, Jinzhou 121013, Peoples R China
Recommended Citation
GB/T 7714
Hou, Shuhua,Ji, Debin,Liu, Wujun,et al. Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2014,24(5):1307-1309.
APA Hou, Shuhua,Ji, Debin,Liu, Wujun,Wang, Lei,&Zhao, Zongbao .(2014).Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs.BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,24(5),1307-1309.
MLA Hou, Shuhua,et al."Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs".BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 24.5(2014):1307-1309.
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