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题名: Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs
作者: Hou, Shuhua1, 2, 3;  Ji, Debin1, 2;  Liu, Wujun1, 2;  Wang, Lei1, 2;  Zhao, Zongbao (Kent)1, 2
关键词: Cofactor ;  Malic enzyme ;  Nicotinamide adenine dinucleotide ;  Orthogonal redox system ;  Enzyme kinetics
刊名: BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
发表日期: 2014-03-01
DOI: 10.1016/j.bmcl.2014.01.047
卷: 24, 期:5, 页:1307-1309
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Chemistry, Medicinal ;  Chemistry, Organic
研究领域[WOS]: Pharmacology & Pharmacy ;  Chemistry
英文摘要: An activity screening between 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide (NAD) analogs and malic enzyme (ME) mutants identified some mutants capable of taking NAD analogs as the cofactor. One particular pair, ME-L310K/L404S and the analog B-8 had good catalytic efficiency and cofactor specificity. The new system gained about 1200-fold cofactor specificity shift from NAD toward B-8 in terms of oxidative decarboxylation of L-malate. Our results provided insightful information for the development of orthogonal redox system that is of particular important to precisely control engineered metabolic pathways. (C) 2014 Elsevier Ltd. All rights reserved.
关键词[WOS]: BIOORTHOGONAL CHEMISTRY ;  SMALL MOLECULES ;  NAD ANALOGS ;  DISCRIMINATION ;  DEHYDROGENASE ;  SPECIFICITY ;  SYSTEMS ;  LIFE
语种: 英语
WOS记录号: WOS:000331705200012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145771
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Div Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, State Key Lab Catalysis, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Bohai Univ, Dept Chem, Liaoning Key Lab Synth & Applicat Funct Cpds, Jinzhou 121013, Peoples R China

Recommended Citation:
Hou, Shuhua,Ji, Debin,Liu, Wujun,et al. Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2014,24(5):1307-1309.
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