DICP OpenIR
Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs
Hou, Shuhua1,2,3; Ji, Debin1,2; Liu, Wujun1,2; Wang, Lei1,2; Zhao, Zongbao (Kent)1,2
关键词Cofactor Malic Enzyme Nicotinamide Adenine Dinucleotide Orthogonal Redox System Enzyme Kinetics
刊名BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
2014-03-01
DOI10.1016/j.bmcl.2014.01.047
24期:5页:1307-1309
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Chemistry, Medicinal ; Chemistry, Organic
研究领域[WOS]Pharmacology & Pharmacy ; Chemistry
关键词[WOS]BIOORTHOGONAL CHEMISTRY ; SMALL MOLECULES ; NAD ANALOGS ; DISCRIMINATION ; DEHYDROGENASE ; SPECIFICITY ; SYSTEMS ; LIFE
英文摘要An activity screening between 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide (NAD) analogs and malic enzyme (ME) mutants identified some mutants capable of taking NAD analogs as the cofactor. One particular pair, ME-L310K/L404S and the analog B-8 had good catalytic efficiency and cofactor specificity. The new system gained about 1200-fold cofactor specificity shift from NAD toward B-8 in terms of oxidative decarboxylation of L-malate. Our results provided insightful information for the development of orthogonal redox system that is of particular important to precisely control engineered metabolic pathways. (C) 2014 Elsevier Ltd. All rights reserved.
语种英语
WOS记录号WOS:000331705200012
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145771
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Div Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, State Key Lab Catalysis, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Bohai Univ, Dept Chem, Liaoning Key Lab Synth & Applicat Funct Cpds, Jinzhou 121013, Peoples R China
推荐引用方式
GB/T 7714
Hou, Shuhua,Ji, Debin,Liu, Wujun,et al. Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2014,24(5):1307-1309.
APA Hou, Shuhua,Ji, Debin,Liu, Wujun,Wang, Lei,&Zhao, Zongbao .(2014).Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs.BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,24(5),1307-1309.
MLA Hou, Shuhua,et al."Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs".BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 24.5(2014):1307-1309.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Hou, Shuhua]的文章
[Ji, Debin]的文章
[Liu, Wujun]的文章
百度学术
百度学术中相似的文章
[Hou, Shuhua]的文章
[Ji, Debin]的文章
[Liu, Wujun]的文章
必应学术
必应学术中相似的文章
[Hou, Shuhua]的文章
[Ji, Debin]的文章
[Liu, Wujun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。