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题名: Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling
作者: Sun, Huanhuan1;  Wang, Lei1;  Zhang, Baoqin2;  Ma, Junhong3;  Hettenhausen, Christian1;  Cao, Guoyan1;  Sun, Guiling1;  Wu, Jianqiang1;  Wu, Jinsong1
关键词: Alternaria alternata ;  feruloyl-CoA 6 '-hydroxylase 1 (F6 ' H1) ;  jasmonic acid (JA) ;  MYC2 ;  Nicotiana attenuata ;  scopoletin ;  virus-induced gene silencing (VIGS)
刊名: JOURNAL OF EXPERIMENTAL BOTANY
发表日期: 2014-08-01
DOI: 10.1093/jxb/eru203
卷: 65, 期:15, 页:4305-4315
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Plant Sciences
研究领域[WOS]: Plant Sciences
英文摘要: Alternaria alternata (tobacco pathotype) is a necrotrophic fungus causing severe losses in Nicotiana species by infection of mature leaves. Similar to what has been observed in cultivated tobacco, N. tabacum, young leaves of wild tobacco, N. attenuata, were more resistant to A. alternata than mature leaves, and this was correlated with stronger blue fluorescence induced after infection. However, the nature of the fluorescence-emitting compound, its role in defence, and its regulation were not clear. Silencing feruloyl-CoA 6'-hydroxylase 1 (F6'H1), the gene encoding the key enzyme for scopoletin biosynthesis, by virus-induced gene silencing (VIGS) revealed that the blue fluorescence was mainly emitted by scopoletin and its beta-glycoside form, scopolin. Further analysis showed that scopoletin exhibited strong antifungal activity against A. alternata in vitro and in vivo. Importantly, jasmonic acid (JA) levels were highly elicited in young leaves but much less in mature leaves after infection; and fungus-elicited scopoletin was absent in JA-deficient plants, but was largely restored with methyl jasmonate treatments. Consistent with this, plants strongly impaired in JA biosynthesis and perception were highly susceptible to A. alternata in the same way scopoletin/scopolin-depleted VIGS F6'H1 plants. Furthermore, silencing MYC2, a master regulator of most JA responses, reduced A. alternata-induced NaF6'H1 transcripts and scopoletin. Thus, it is concluded that JA signalling is activated in N. attenuata leaves after infection, which subsequently regulates scopoletin biosynthesis for the defence against A. alternata partly through MYC2, and higher levels of scopoletin accumulated in young leaves account for their strong resistance.
关键词[WOS]: TRYPSIN PROTEINASE-INHIBITORS ;  NICOTIANA-ATTENUATA ;  PSEUDOMONAS-SYRINGAE ;  ARABIDOPSIS-THALIANA ;  HEVEA-BRASILIENSIS ;  DEFENSE RESPONSES ;  TRANSCRIPTION FACTORS ;  HERBIVORE RESISTANCE ;  DISEASE RESISTANCE ;  METHYL JASMONATE
语种: 英语
WOS记录号: WOS:000339954300016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145773
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Yunnan Acad Tobacco Agr Sci, Kunming 650031, Peoples R China

Recommended Citation:
Sun, Huanhuan,Wang, Lei,Zhang, Baoqin,et al. Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling[J]. JOURNAL OF EXPERIMENTAL BOTANY,2014,65(15):4305-4315.
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