中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells
作者: Liu, Zhao-Ming1, 4;  Feng, Lei2;  Hou, Jie3;  Lv, Xia1;  Ning, Jing1, 3;  Ge, Guang-Bo1;  Wang, Ke-Wei2;  Cui, Jing-Nan2;  Yang, Ling1
关键词: Human carboxylesterase 2 ;  Ratiometric fluorescent probe ;  4-Hydroxy-N-butyl-1,8-naphthalimide ;  Bioimaging
刊名: SENSORS AND ACTUATORS B-CHEMICAL
发表日期: 2014-12-15
DOI: 10.1016/j.snb.2014.08.066
卷: 205, 页:151-157
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Analytical ;  Electrochemistry ;  Instruments & Instrumentation
研究领域[WOS]: Chemistry ;  Electrochemistry ;  Instruments & Instrumentation
英文摘要: A new ratiometric fluorescent probe derived from 4-hydroxy-N-butyl-1,8-naphthalimide (HNN) has been developed for selective detection of human carboxylesterase 2 (hCE2). The probe is designed by introducing benzoyl moiety to HNN, based on the intramolecular charge transfer (ICT) mechanism. The probe displays satisfying stability under physiological pH conditions with very low background fluorescence signal, but it can be rapidly hydrolyzed by hCE2 and release of HNN which leads to a remarkable red shift in emission spectra (148 nm). The newly designed probe exhibits excellent selectivity towards hCE2 over other human hydrolases, while the interference from various biologically relevant chemicals can be negligible. Its potential biological applications including inhibitor screening using human tissue preparations as enzyme sources, as well as fluorescence imaging of endogenous hCE2 in human living cells, have also been demonstrated. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: HUMAN INTESTINAL CARBOXYLESTERASE ;  MAMMALIAN CARBOXYLESTERASES ;  HUMAN LIVER ;  CATALYTIC-PROPERTIES ;  CPT-11 HYDROLYSIS ;  TUMOR-TISSUE ;  PROBE ;  IRINOTECAN ;  INHIBITORS ;  METABOLISM
语种: 英语
WOS记录号: WOS:000343117600020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145788
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116024, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
3.Dalian Med Univ, Dalian 116044, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

Recommended Citation:
Liu, Zhao-Ming,Feng, Lei,Hou, Jie,et al. A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,205:151-157.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Liu, Zhao-Ming]'s Articles
 [Feng, Lei]'s Articles
 [Hou, Jie]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Liu, Zhao-Ming]‘s Articles
 [Feng, Lei]‘s Articles
 [Hou, Jie]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace