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A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells
Liu, Zhao-Ming1,4; Feng, Lei2; Hou, Jie3; Lv, Xia1; Ning, Jing1,3; Ge, Guang-Bo1; Wang, Ke-Wei2; Cui, Jing-Nan2; Yang, Ling1
关键词Human Carboxylesterase 2 Ratiometric Fluorescent Probe Bioimaging 4-hydroxy-n-butyl-1 8-naphthalimide
刊名SENSORS AND ACTUATORS B-CHEMICAL
2014-12-15
DOI10.1016/j.snb.2014.08.066
205页:151-157
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
研究领域[WOS]Chemistry ; Electrochemistry ; Instruments & Instrumentation
关键词[WOS]HUMAN INTESTINAL CARBOXYLESTERASE ; MAMMALIAN CARBOXYLESTERASES ; HUMAN LIVER ; CATALYTIC-PROPERTIES ; CPT-11 HYDROLYSIS ; TUMOR-TISSUE ; PROBE ; IRINOTECAN ; INHIBITORS ; METABOLISM
英文摘要A new ratiometric fluorescent probe derived from 4-hydroxy-N-butyl-1,8-naphthalimide (HNN) has been developed for selective detection of human carboxylesterase 2 (hCE2). The probe is designed by introducing benzoyl moiety to HNN, based on the intramolecular charge transfer (ICT) mechanism. The probe displays satisfying stability under physiological pH conditions with very low background fluorescence signal, but it can be rapidly hydrolyzed by hCE2 and release of HNN which leads to a remarkable red shift in emission spectra (148 nm). The newly designed probe exhibits excellent selectivity towards hCE2 over other human hydrolases, while the interference from various biologically relevant chemicals can be negligible. Its potential biological applications including inhibitor screening using human tissue preparations as enzyme sources, as well as fluorescence imaging of endogenous hCE2 in human living cells, have also been demonstrated. (C) 2014 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000343117600020
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145788
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116024, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
3.Dalian Med Univ, Dalian 116044, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
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Liu, Zhao-Ming,Feng, Lei,Hou, Jie,et al. A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,205:151-157.
APA Liu, Zhao-Ming.,Feng, Lei.,Hou, Jie.,Lv, Xia.,Ning, Jing.,...&Yang, Ling.(2014).A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells.SENSORS AND ACTUATORS B-CHEMICAL,205,151-157.
MLA Liu, Zhao-Ming,et al."A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells".SENSORS AND ACTUATORS B-CHEMICAL 205(2014):151-157.
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