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Nontargeted Modification-Specific Metabolomics Study Based on Liquid Chromatography High-Resolution Mass Spectrometry
Dai, Weidong1; Yin, Peiyuan1; Zeng, Zhongda1; Kong, Hongwei1; Tong, Hongwei2; Xu, Zhiliang1; Lu, Xin1; Lehmann, Rainer3,4,5; Xu, Guowang1
Source PublicationANALYTICAL CHEMISTRY
2014-09-16
DOI10.1021/ac502045j
Volume86Issue:18Pages:9146-9153
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMODIFICATION-SPECIFIC PROTEOMICS ; POSTTRANSLATIONAL MODIFICATIONS ; HUMAN URINE ; METABOLISM ; CANCER ; IDENTIFICATION ; ACETYLATION ; STRATEGIES ; ENRICHMENT ; DIAGNOSIS
AbstractModifications of genes and proteins have been extensively studied in systems biology using comprehensive analytical strategies. Although metabolites are frequently modified, these modifications have not been studied using -omics approaches. Here a general strategy for the nontargeted profiling of modified metabolites, which we call nontargeted modification-specific metabolomics, is reported. A key aspect of this strategy was the combination of in-source collision-induced dissociation liquid chromatographymass spectrometry (LC-MS) and global nontargeted LC-MS-based metabolomics. Characteristic neutral loss fragments that are specific for acetylation, sulfation, glucuronidation, glucosidation, or ribose conjugation were reproducibly detected using human urine as a model specimen for method development. The practical application of this method was demonstrated by profiling urine samples from liver cirrhosis patients. Approximately 900 features were identified as modified endogenous metabolites and xenobiotics. Moreover, this strategy supports the identification of compounds not included in traditional metabolomics databases (HMDB, Metlin, and KEGG), which are currently referred to as unknowns in metabolomics projects. Nontargeted modification-specific metabolomics opens a new perspective in systems biology.
Language英语
WOS IDWOS:000341801200036
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145804
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Zhejiang Chinese Med Univ, Affiliated Hosp 1, Hangzhou 310006, Zhejiang, Peoples R China
3.Univ Hosp Tuebingen, Dept Internal Med 4, Div Clin Chem & Pathobiochem, D-72076 Tubingen, Germany
4.Univ Tubingen, Helmholtz Ctr Munich, Inst Diabet Res & Metab Dis, D-72076 Tubingen, Germany
5.German Ctr Diabet Res DZD, D-72076 Tubingen, Germany
Recommended Citation
GB/T 7714
Dai, Weidong,Yin, Peiyuan,Zeng, Zhongda,et al. Nontargeted Modification-Specific Metabolomics Study Based on Liquid Chromatography High-Resolution Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2014,86(18):9146-9153.
APA Dai, Weidong.,Yin, Peiyuan.,Zeng, Zhongda.,Kong, Hongwei.,Tong, Hongwei.,...&Xu, Guowang.(2014).Nontargeted Modification-Specific Metabolomics Study Based on Liquid Chromatography High-Resolution Mass Spectrometry.ANALYTICAL CHEMISTRY,86(18),9146-9153.
MLA Dai, Weidong,et al."Nontargeted Modification-Specific Metabolomics Study Based on Liquid Chromatography High-Resolution Mass Spectrometry".ANALYTICAL CHEMISTRY 86.18(2014):9146-9153.
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