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题名: Preparation and characterization of vorinostat-coated beads for profiling of novel target proteins
作者: Lu, Congcong1, 2;  Zhang, Kai1, 2, 3, 4;  Zhang, Yi5, 6;  Tan, Minjia5, 6;  Li, Yijun1, 2;  He, Xiwen1, 2;  Zhang, Yukui1, 2, 7
关键词: Proteomics ;  HPLC-MS/MS ;  Chemical probe ;  Target proteins ;  Vorinostat
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-12-12
DOI: 10.1016/j.chroma.2014.10.098
卷: 1372, 页:34-41
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Inhibitors of histone deacetylases (HDACs) have been considered to be new anticancer agents. As a key inhibitor of HDAC, vorinostat can cause growth arrest and death of a broad of transformed cells and interact with a variety of substrates. A comprehensive analysis of proteins interacting with HDAC inhibitors is of great importance in understanding molecular mechanisms of the drugs. Here, we reported the preparation and characterization of vorinostat-coated beads for profiling of novel target proteins of vorinostat (a key HDAC inhibitor). The enriched proteins were further analyzed by HPLC-MS/MS. Besides the known substrates, there were also several novel enriched protein candidates, one of which was a metalloenzyme a-enolase (ENO-1). According to our best knowledge, it is the first time that ENO-1 has been detected as a potential target of vorinostat through chemoproteomics approach. Further competition analysis indicated that ENO-1 may be co-enriched as a substrate complex. Our results demonstrated that the chemical probe combined with proteomics approach may be developed as a potential tool to identify target proteins of drugs. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: HISTONE DEACETYLASE INHIBITORS ;  LYSINE ACETYLATION ;  MOLECULAR-MECHANISMS ;  HYDROXAMIC ACID ;  CANCER CELLS ;  HDAC ;  SAHA ;  THERAPIES ;  COMPLEXES
语种: 英语
WOS记录号: WOS:000347020900004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145808
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Tianjin Med Univ, Basic Med Sch, Dept Biochem & Mol Biol, Tianjin 300070, Peoples R China
4.Tianjin Med Univ, Basic Med Sch, Tianjin Key Lab Med Epigenet, Tianjin 300070, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Mat Med, Chem Prote Ctr, Shanghai 201203, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
7.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Lu, Congcong,Zhang, Kai,Zhang, Yi,et al. Preparation and characterization of vorinostat-coated beads for profiling of novel target proteins[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1372:34-41.
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