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Transcript profiling analysis of in vitro cultured THP-1 cells after exposure to crotonaldehyde
Yang, Bi-cheng1,2; Yang, Zhi-hua1; Pan, Xiu-jie1; Wang, Li-meng2; Liu, Xing-yu4; Zhu, Mao-xiang1; Xie, Jian-ping3
KeywordCrotonaldehyde Macrophages Digital Gene Expression
Source PublicationJOURNAL OF TOXICOLOGICAL SCIENCES
2014-06-01
Volume39Issue:3Pages:487-497
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectToxicology
WOS Research AreaToxicology
WOS KeywordBRONCHIAL EPITHELIAL-CELLS ; NF-KAPPA-B ; CIGARETTE-SMOKE ; GENE-EXPRESSION ; ALVEOLAR MACROPHAGES ; ALPHA,BETA-UNSATURATED ALDEHYDES ; SIGNALING PATHWAYS ; OXIDATIVE STRESS ; IMMUNE-RESPONSE ; IL-8 RELEASE
AbstractCrotonaldehyde, a highly toxic alpha, beta-unsaturated aldehyde, is a major component of cigarette smoke and a ubiquitous environmental pollutant. Crotonaldehyde exposure is known to have adverse effects on respiratory health, but the underlying mechanisms remain obscure. As alveolar macrophages display important immunological and inflammatory properties in response to extraneous substances in the lung, we aimed at gaining more insight in changes of human macrophage-like cells transcriptome in response to crotonaldehyde. In vitro cultures of human THP-1 cells (a human monocytic leukemia cell line) were differentiated into macrophage-like cells treated by PMA (phorbol 12-myristate 13-acetate) and be exposed crotonaldehyde. Using RNA-seq technology such as digital gene expression, the global changes in transcriptional level were analyzed. Real-time quantitative polymerase chain reaction (qPCR) was performed to validate RNA-seq data. The differential regulated genes in many biological processes were dysregulated, including in antigen processing and presentation, oxidative stress, inflammation, cytokine signaling, and apoptosis. Collectively, our study demonstrated that crotonaldehyde altered gene expression profile in the genome-wide transcriptional level in human macrophage-like cells, and many of them may represent potential mechanisms of crotonaldehyde causing cytotoxicity and tissue injury in the human lung.
Language英语
WOS IDWOS:000337069200012
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145819
Collection中国科学院大连化学物理研究所
Affiliation1.Beijing Inst Radiat Med, Dept Radiat Toxicol & Oncol, Beijing 100850, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
3.Zhengzhou Tobacco Res Inst CNTC, Zhengzhou 450001, Henan, Peoples R China
4.Shanghai Tobacco Corp, Ctr Technol, Beijing Work Stn, Beijing 101121, Peoples R China
Recommended Citation
GB/T 7714
Yang, Bi-cheng,Yang, Zhi-hua,Pan, Xiu-jie,et al. Transcript profiling analysis of in vitro cultured THP-1 cells after exposure to crotonaldehyde[J]. JOURNAL OF TOXICOLOGICAL SCIENCES,2014,39(3):487-497.
APA Yang, Bi-cheng.,Yang, Zhi-hua.,Pan, Xiu-jie.,Wang, Li-meng.,Liu, Xing-yu.,...&Xie, Jian-ping.(2014).Transcript profiling analysis of in vitro cultured THP-1 cells after exposure to crotonaldehyde.JOURNAL OF TOXICOLOGICAL SCIENCES,39(3),487-497.
MLA Yang, Bi-cheng,et al."Transcript profiling analysis of in vitro cultured THP-1 cells after exposure to crotonaldehyde".JOURNAL OF TOXICOLOGICAL SCIENCES 39.3(2014):487-497.
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