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题名: Transcript profiling analysis of in vitro cultured THP-1 cells after exposure to crotonaldehyde
作者: Yang, Bi-cheng1, 2;  Yang, Zhi-hua1;  Pan, Xiu-jie1;  Wang, Li-meng2;  Liu, Xing-yu4;  Zhu, Mao-xiang1;  Xie, Jian-ping3
关键词: Crotonaldehyde ;  Macrophages ;  Digital gene expression
刊名: JOURNAL OF TOXICOLOGICAL SCIENCES
发表日期: 2014-06-01
卷: 39, 期:3, 页:487-497
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
英文摘要: Crotonaldehyde, a highly toxic alpha, beta-unsaturated aldehyde, is a major component of cigarette smoke and a ubiquitous environmental pollutant. Crotonaldehyde exposure is known to have adverse effects on respiratory health, but the underlying mechanisms remain obscure. As alveolar macrophages display important immunological and inflammatory properties in response to extraneous substances in the lung, we aimed at gaining more insight in changes of human macrophage-like cells transcriptome in response to crotonaldehyde. In vitro cultures of human THP-1 cells (a human monocytic leukemia cell line) were differentiated into macrophage-like cells treated by PMA (phorbol 12-myristate 13-acetate) and be exposed crotonaldehyde. Using RNA-seq technology such as digital gene expression, the global changes in transcriptional level were analyzed. Real-time quantitative polymerase chain reaction (qPCR) was performed to validate RNA-seq data. The differential regulated genes in many biological processes were dysregulated, including in antigen processing and presentation, oxidative stress, inflammation, cytokine signaling, and apoptosis. Collectively, our study demonstrated that crotonaldehyde altered gene expression profile in the genome-wide transcriptional level in human macrophage-like cells, and many of them may represent potential mechanisms of crotonaldehyde causing cytotoxicity and tissue injury in the human lung.
关键词[WOS]: BRONCHIAL EPITHELIAL-CELLS ;  NF-KAPPA-B ;  CIGARETTE-SMOKE ;  GENE-EXPRESSION ;  ALVEOLAR MACROPHAGES ;  ALPHA,BETA-UNSATURATED ALDEHYDES ;  SIGNALING PATHWAYS ;  OXIDATIVE STRESS ;  IMMUNE-RESPONSE ;  IL-8 RELEASE
语种: 英语
WOS记录号: WOS:000337069200012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145819
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Beijing Inst Radiat Med, Dept Radiat Toxicol & Oncol, Beijing 100850, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
3.Zhengzhou Tobacco Res Inst CNTC, Zhengzhou 450001, Henan, Peoples R China
4.Shanghai Tobacco Corp, Ctr Technol, Beijing Work Stn, Beijing 101121, Peoples R China

Recommended Citation:
Yang, Bi-cheng,Yang, Zhi-hua,Pan, Xiu-jie,et al. Transcript profiling analysis of in vitro cultured THP-1 cells after exposure to crotonaldehyde[J]. JOURNAL OF TOXICOLOGICAL SCIENCES,2014,39(3):487-497.
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