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题名: Bacillus amyloliquefaciens Q-426 as a potential biocontrol agent against Fusarium oxysporum f. sp spinaciae
作者: Zhao, Pengchao1, 2;  Quan, Chunshan3;  Wang, Yingguo1, 2;  Wang, Jianhua1, 2;  Fan, Shengdi3
关键词: Gene clusters ;  Lipopeptides ;  Fungistatic agent ;  Fengycin A ;  Antifungal mechanism
刊名: JOURNAL OF BASIC MICROBIOLOGY
发表日期: 2014-05-01
DOI: 10.1002/jobm.201200414
卷: 54, 期:5, 页:448-456
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
英文摘要: In recent years, Bacillus species have received considerable attention for the biological control of many fungal diseases. In this study, Bacillus amyloliquefaciens Q-426 was tested for its potential use against a variety of plant pathogens. Our screen for genes involved in the biosynthesis of antifungal agents revealed that the fen and bmy gene clusters are present in the Q-426 genome. Lipopeptides such as bacillomycin D, fengycin A, and fengycin B were purified from the bacterial culture broth and subsequently identified by ESI-mass spectrometry. The minimal inhibitory concentration of fengycin A against Fusarium oxysporum f. sp. spinaciae W.C. Snyder & H.N. Hansen O-27 was determined to be 31.25gml(-1). However, exposure of fungal cells to 50gml(-1) of fengycin A did not allow permeation of fluorescein diacetate into the cytoplasm through the cell membrane. Moreover, leakage of intracellular inorganic cations, nucleic acid and protein were also not detected, indicating that the fungal cell membrane is not the primary target of action for fengycin A. Profound morphological changes were observed in the F. oxysporum strain and spore germination was completely inhibited, suggesting that 50gml(-1) of fengycin A acts, at least, as a fungistatic agent.
关键词[WOS]: NONRIBOSOMAL SYNTHESIS ;  ANTIFUNGAL ACTIVITY ;  ITURIN-A ;  SUBTILIS ;  LIPOPEPTIDES ;  SURFACTIN ;  FENGYCIN ;  MECHANISM ;  REVEALS ;  SOIL
语种: 英语
WOS记录号: WOS:000336216700013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145837
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Dalian Nationalities Univ, Dept Life Sci, Dalian, Peoples R China

Recommended Citation:
Zhao, Pengchao,Quan, Chunshan,Wang, Yingguo,et al. Bacillus amyloliquefaciens Q-426 as a potential biocontrol agent against Fusarium oxysporum f. sp spinaciae[J]. JOURNAL OF BASIC MICROBIOLOGY,2014,54(5):448-456.
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