中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Study of metabolite differences of flue-cured tobacco from different regions using a pseudotargeted gas chromatography with mass spectrometry selected-ion monitoring method
作者: Zhao, Yanni1;  Zhao, Chunxia1;  Li, Yanli1;  Chang, Yuwei1;  Zhang, Junjie1;  Zeng, Zhongda1;  Lu, Xin1;  Xu, Guowang1
关键词: Flue-cured tobacco ;  Gas chromatography with mass spectrometry ;  Pseudotargeted analysis ;  Selected-ion monitoring
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2014-08-01
DOI: 10.1002/jssc.201400097
卷: 37, 期:16, 页:2177-2184
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A pseudotargeted method based on gas chromatography and mass spectrometry with selected-ion monitoring was established to investigate the metabolite differences of flue-cured tobacco from three different growing regions. The mixed solvent of acetonitrile/isopropanol/water (3:3:2, v/v/v) was chosen as the optimal extraction system based on the good repeatability and extraction efficiency. A self-developed software coupled with commercial software was used to establish the pseudotargeted method including 289 peaks and 47 groups. Multivariable statistical analysis indicated that tobacco samples can be obviously separated based on the geographical origins. On the basis of a Mann-Whitney U test, organic acids, phenols, and alkaloids had higher levels in Hunan province. In contrast, a large proportion of amino acids (including L-tyrosine, L-proline, and serine), sucrose, and linoleic acid were the highest in Yunnan province. Meanwhile, multiple metabolic pathways (including carbohydrate metabolism, tricarboxylic acid cycle, and nitrogen metabolism) were influenced by growing regions. Twenty-eight differential metabolites, which had great contributions to the classification of tobacco samples of three growing regions, were further defined. The results demonstrated that the developed pseudotargeted method was a powerful tool to investigate the metabolic profiling of tobacco leaves and discriminate tobacco leaves of different growing regions.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  VOLATILE ORGANIC-ACIDS ;  GC-MS ;  TRANSGENIC RICE ;  QUALITY-CONTROL ;  WILD-TYPE ;  REVEALS ;  LEAVES ;  ARABIDOPSIS ;  RESPONSES
语种: 英语
WOS记录号: WOS:000340556300012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145840
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Zhao, Yanni,Zhao, Chunxia,Li, Yanli,et al. Study of metabolite differences of flue-cured tobacco from different regions using a pseudotargeted gas chromatography with mass spectrometry selected-ion monitoring method[J]. JOURNAL OF SEPARATION SCIENCE,2014,37(16):2177-2184.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhao, Yanni]'s Articles
 [Zhao, Chunxia]'s Articles
 [Li, Yanli]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhao, Yanni]‘s Articles
 [Zhao, Chunxia]‘s Articles
 [Li, Yanli]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace