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Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry
Zhu, Jun1; Sun, Zhen1; Cheng, Kai1; Chen, Rui1; Ye, Mingliang1; Xu, Bo1; Sun, Deguang2; Wang, Liming2; Liu, Jing1; Wang, Fangjun1; Zou, Hanfa1
KeywordN-glycosylation Sites N-glycoproteins Hilic Hydrazide Chemistry Human Liver
Source PublicationJOURNAL OF PROTEOME RESEARCH
2014-03-01
DOI10.1021/pr401200h
Volume13Issue:3Pages:1713-1721
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordMASS-SPECTROMETRY ; HEPATOCELLULAR-CARCINOMA ; GLYCOPEPTIDE CAPTURE ; STELLATE CELLS ; KAPPA-B ; DATABASE ; IDENTIFICATION ; FIBROSIS ; HETEROGENEITY ; ORGANIZATION
AbstractAlthough glycoproteomics is greatly developed in recent years, our knowledge about N-glycoproteome of human tissues is still very limited. In this study, we comprehensively mapped the N-glycosylation sites of human liver by combining click maltose-hydrophilic interaction chromatography (HILIC) and the improved hydrazide chemistry. The specificity could be as high as 90% for hydrazide chemistry and 80% for HILIC. Altogether, we identified 14 480 N-glycopeptides matched with N-!P-[SITIC] sequence motif from human liver, corresponding to 2210 N-glycoproteins and 4783 N-glycosylation sites. These N-glycoproteins are widely involved into different types of biological processes, such as hepatic stellate cell activation and acute phase response of human liver, which all highly associate with the progression of liver diseases. Moreover, the exact N-glycosylation sites of some key-regulating proteins within different human liver physiological processes were also obtained, such as E-cadherin, transforming growth factor beta receptor and 29 members of G protein coupled receptors family.
Language英语
WOS IDWOS:000332756300049
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/145847
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Jun,Sun, Zhen,Cheng, Kai,et al. Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(3):1713-1721.
APA Zhu, Jun.,Sun, Zhen.,Cheng, Kai.,Chen, Rui.,Ye, Mingliang.,...&Zou, Hanfa.(2014).Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry.JOURNAL OF PROTEOME RESEARCH,13(3),1713-1721.
MLA Zhu, Jun,et al."Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry".JOURNAL OF PROTEOME RESEARCH 13.3(2014):1713-1721.
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