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题名: Protein-protein interaction network of the marine microalga Tetraselmis subcordiformis: prediction and application for starch metabolism analysis
作者: Ji, Chaofan1;  Cao, Xupeng2;  Yao, Changhong2;  Xue, Song2;  Xiu, Zhilong1
关键词: Chlorophyceae ;  Interolog ;  Ortholog ;  Post-translational modification
刊名: JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
发表日期: 2014-08-01
DOI: 10.1007/s10295-014-1462-z
卷: 41, 期:8, 页:1287-1296
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Under stressful conditions, the non-model marine microalga Tetraselmis subcordiformis can accumulate a substantial amount of starch, making it a potential feedstock for the production of fuel ethanol. Investigating the interactions of the enzymes and the regulatory factors involved in starch metabolism will provide potential genetic manipulation targets for optimising the starch productivity of T. subcordiformis. For this reason, the proteome of T. subcordiformis was utilised to predict the first protein-protein interaction (PPI) network for this marine alga based on orthologous interactions, mainly from the general PPI repositories. Different methods were introduced to evaluate the credibility of the predicted interactome, including the confidence value of each PPI pair and Pfam-based and subcellular location-based enrichment analysis. Functional subnetworks analysis suggested that the two enzymes involved in starch metabolism, starch phosphorylase and trehalose-phosphate synthase may be the potential ideal genetic engineering targets.
关键词[WOS]: BIOSYNTHETIC-ENZYMES ;  INTERACTION DATABASE ;  AMYLOPLASTS ;  COMPLEXES ;  ASSOCIATE
语种: 英语
WOS记录号: WOS:000339385400012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145854
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China

Recommended Citation:
Ji, Chaofan,Cao, Xupeng,Yao, Changhong,et al. Protein-protein interaction network of the marine microalga Tetraselmis subcordiformis: prediction and application for starch metabolism analysis[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2014,41(8):1287-1296.
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