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题名: Neural activity analysis of pure chito-oligomer components separated from a mixture of chitooligosaccharides
作者: Jiang, Maorong1;  Guo, Zhimou2;  Wang, Caiping1;  Yang, Yuming1;  Liang, Xinmiao2;  Ding, Fei1
关键词: Chitooligosaccharides ;  Chito-oligomer ;  Schwann cell ;  Dorsal root ganglion (DRG) ;  Neurite outgrowth
刊名: NEUROSCIENCE LETTERS
发表日期: 2014-10-03
DOI: 10.1016/j.neulet.2014.08.014
卷: 581, 页:32-36
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
英文摘要: Chitooligosaccharides (COSs) are obtained from the in vivo and in vitro degradation of chitosan, which is a natural biomaterial used successfully for peripheral nerve repair. Also, COSs have been reported to be beneficial for the nervous system and especially able to promote peripheral nerve regeneration. To determine which component in a mixture of COSs was really responsible for the neurotrophic action of COSs, this study was performed to separate the active components of COSs and compare the neural activity of different pure components. Hydrophilic interaction liquid chromatography (HILIC) was used to separate 5 chito-oligomers with single degrees of polymerization (DPs) from a mixture of COSs. They were chitobiose, chitotriose, chitotetraose, chitopentaose, and chitohexaose with DPs of 2-6, respectively. MTT assay indicated that chitotriose induced the greatest increase in Schwann cell survival among 5 chito-oligomers. Immunocytochemistry with anti-NF-200 showed that chitotriose significantly encouraged neurite outgrowth from dorsal root ganglion (DRG) explants with the greatest effect among 5 chito-oligomers. Our results suggest that chitotriose may be a key component in COSs mixture. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
关键词[WOS]: MOLECULAR-WEIGHT CHITOSAN ;  NEURITE OUTGROWTH ;  NERVE GRAFTS ;  IN-VITRO ;  NEURONS ;  CELLS ;  ACID ;  REGENERATION ;  PATHWAY ;  COS
语种: 英语
WOS记录号: WOS:000342872500006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145871
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nantong Univ, Jiangsu Key Lab Neuroregenerat, Nantong 226001, JS, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, LN, Peoples R China

Recommended Citation:
Jiang, Maorong,Guo, Zhimou,Wang, Caiping,et al. Neural activity analysis of pure chito-oligomer components separated from a mixture of chitooligosaccharides[J]. NEUROSCIENCE LETTERS,2014,581:32-36.
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