中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Evaluation of the peak variance in gradient liquid chromatography
作者: Hao, Weiqiang1, 2;  Di, Bin3;  Chen, Qiang1;  Wang, Junde4;  Yang, Yongbing1;  Yue, Bangyi1
刊名: ANALYST
发表日期: 2014
DOI: 10.1039/c3an02030d
卷: 139, 期:6, 页:1504-1511
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The peak variance obtained under single stepwise (SS), single linear (SL) and ladder-like (LL) gradient conditions was evaluated by taking into account the variation of plate height with mobile phase composition. LC tests were performed on a C18 column using a mixture of methanol and water as the mobile phase and six aromatic compounds (anisole, o-cresol, biphenyl, phenol, aniline and acetophenone) as the model samples. It was found that the relationship between the retention factor and mobile phase composition could be described by the linear solvent strength model. The plate height that was measured under isocratic conditions varied with mobile phase composition. This variation could be described by a quadratic polynomial in this work. Under gradient conditions, the experimental and predicted values of peak variance were found to be well consistent. The discrepancy between the experimental and predicted values in the SS gradient was larger than that in the SL or LL gradient. This may be ascribed to the distortion of the gradient profile as a result of dispersion that occurred in the mobile phase. By using the dispersive gradient profile that was obtained at the column inlet for the calculation, the discrepancy between the experimental and predicted values in the SS gradient may be minimized.
关键词[WOS]: MASS-TRANSFER ;  ELUTION CHROMATOGRAPHY ;  COMPRESSION FACTOR ;  ORGANIC MODIFIER ;  MODEL ;  RETENTION ;  OPTIMIZATION ;  BANDWIDTH ;  PREDICT ;  WIDTH
语种: 英语
WOS记录号: WOS:000331867500031
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145894
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Nanjing Univ, High Tech Res Inst, Changzhou 213164, Peoples R China
2.Shanghai Tobacco Grp Co LTD, Shanghai Tobacco Grp, CNTC, Key Lab Cigarette Smoke Res, Shanghai 200082, Peoples R China
3.China Pharmaceut Univ, Dept Pharmaceut Anal, Nanjing 210009, Jiangsu, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Hao, Weiqiang,Di, Bin,Chen, Qiang,et al. Evaluation of the peak variance in gradient liquid chromatography[J]. ANALYST,2014,139(6):1504-1511.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Hao, Weiqiang]'s Articles
 [Di, Bin]'s Articles
 [Chen, Qiang]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Hao, Weiqiang]‘s Articles
 [Di, Bin]‘s Articles
 [Chen, Qiang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace