DICP OpenIR
Evaluation of the peak variance in gradient liquid chromatography
Hao, Weiqiang1,2; Di, Bin3; Chen, Qiang1; Wang, Junde4; Yang, Yongbing1; Yue, Bangyi1
刊名ANALYST
2014
DOI10.1039/c3an02030d
139期:6页:1504-1511
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MASS-TRANSFER ; ELUTION CHROMATOGRAPHY ; COMPRESSION FACTOR ; ORGANIC MODIFIER ; MODEL ; RETENTION ; OPTIMIZATION ; BANDWIDTH ; PREDICT ; WIDTH
英文摘要The peak variance obtained under single stepwise (SS), single linear (SL) and ladder-like (LL) gradient conditions was evaluated by taking into account the variation of plate height with mobile phase composition. LC tests were performed on a C18 column using a mixture of methanol and water as the mobile phase and six aromatic compounds (anisole, o-cresol, biphenyl, phenol, aniline and acetophenone) as the model samples. It was found that the relationship between the retention factor and mobile phase composition could be described by the linear solvent strength model. The plate height that was measured under isocratic conditions varied with mobile phase composition. This variation could be described by a quadratic polynomial in this work. Under gradient conditions, the experimental and predicted values of peak variance were found to be well consistent. The discrepancy between the experimental and predicted values in the SS gradient was larger than that in the SL or LL gradient. This may be ascribed to the distortion of the gradient profile as a result of dispersion that occurred in the mobile phase. By using the dispersive gradient profile that was obtained at the column inlet for the calculation, the discrepancy between the experimental and predicted values in the SS gradient may be minimized.
语种英语
WOS记录号WOS:000331867500031
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145894
专题中国科学院大连化学物理研究所
作者单位1.Nanjing Univ, High Tech Res Inst, Changzhou 213164, Peoples R China
2.Shanghai Tobacco Grp Co LTD, Shanghai Tobacco Grp, CNTC, Key Lab Cigarette Smoke Res, Shanghai 200082, Peoples R China
3.China Pharmaceut Univ, Dept Pharmaceut Anal, Nanjing 210009, Jiangsu, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Hao, Weiqiang,Di, Bin,Chen, Qiang,et al. Evaluation of the peak variance in gradient liquid chromatography[J]. ANALYST,2014,139(6):1504-1511.
APA Hao, Weiqiang,Di, Bin,Chen, Qiang,Wang, Junde,Yang, Yongbing,&Yue, Bangyi.(2014).Evaluation of the peak variance in gradient liquid chromatography.ANALYST,139(6),1504-1511.
MLA Hao, Weiqiang,et al."Evaluation of the peak variance in gradient liquid chromatography".ANALYST 139.6(2014):1504-1511.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Hao, Weiqiang]的文章
[Di, Bin]的文章
[Chen, Qiang]的文章
百度学术
百度学术中相似的文章
[Hao, Weiqiang]的文章
[Di, Bin]的文章
[Chen, Qiang]的文章
必应学术
必应学术中相似的文章
[Hao, Weiqiang]的文章
[Di, Bin]的文章
[Chen, Qiang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。