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题名: An epitope imprinting method on the surface of magnetic nanoparticles for specific recognition of bovine serum album
作者: Zhao, Xiao-Li1, 2;  Li, Dong-Yan1, 2;  He, Xi-Wen1, 2;  Li, Wen-You1, 2;  Zhang, Yu-Kui1, 2, 3
刊名: JOURNAL OF MATERIALS CHEMISTRY B
发表日期: 2014
DOI: 10.1039/c4tb01381f
卷: 2, 期:43, 页:7575-7582
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Materials Science, Biomaterials
研究领域[WOS]: Materials Science
英文摘要: In this study, we reported an epitope imprinting method on the surface of core-shell magnetic nanoparticles (NPs) for the recognition of target bovine serum album (BSA). The epitope was selected as the template molecule from the nonapeptide of surface-exposed C-terminus of BSA. The core-shell magnetic epitope molecularly imprinted polymers (Fe3O4@EMIPs) exhibited a specific capture activity for the corresponding target protein, BSA. The magnetic NPs made it easy to separate the imprinted material from solution by an external magnetic field, and the thin imprinted layer presented fast kinetics for the rebinding of the target protein. Moreover, the Fe3O4@EMIPs could separate BSA from the bovine blood sample. The epitope imprinting approach combined with magnetic NPs provided an easy and fast method for the specific recognition of BSA.
关键词[WOS]: PROTEIN ;  POLYMERS ;  HEMOGLOBIN ;  SEPARATION ;  RESOLUTION ;  PEPTIDES ;  CAPTURE ;  FILM
语种: 英语
WOS记录号: WOS:000343992700012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145901
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Coll Chem, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Coll Chem, Res Ctr Analyt Sci, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zhao, Xiao-Li,Li, Dong-Yan,He, Xi-Wen,et al. An epitope imprinting method on the surface of magnetic nanoparticles for specific recognition of bovine serum album[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(43):7575-7582.
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