DICP OpenIR
Decrease of dynamic range of proteins in human plasma by ampholine immobilized polymer microspheres
Deng, Nan1,2; Zhu, Guijie1; Chen, Yuanbo1,2; Wu, Qi1,2; Liang, Zhen1; Sui, Zhigang1; Zhang, Lihua1; Yang, Kaiguang1; Zhang, Yukui1
关键词Ampholine Fractionation Human Plasma Protein Dynamic Range
刊名ANALYTICA CHIMICA ACTA
2014-05-15
DOI10.1016/j.aca.2014.04.004
826页:43-50
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MULTIDIMENSIONAL LIQUID-CHROMATOGRAPHY ; LECTIN AFFINITY-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; LIGAND LIBRARY TREATMENT ; HUMAN SERUM PROTEOME ; EXCHANGE CHROMATOGRAPHY ; BIOMARKER DISCOVERY ; GEL ELECTROPHORESIS ; PEPTIDE LIBRARIES ; REVERSED-PHASE
英文摘要A novel protein sample pretreatment method based on ampholine immobilized polymer microsphere (ampholine@PM) was developed for the fractionation of intact proteins prior to protein digestion and peptide analysis to reduce the dynamic range of human plasma proteome. After incubation with our prepared ampholine@PM, the captured plasma proteins were successively desorbed by 2 M NaCl, 100 mM glycine-hydrochloric acid, and 30% (v/v) acetonitrile with 0.1% (v/v) trifluoroacetic acid. The SDS-PAGE results showed the protein dynamic range in such three fractions was obviously reduced as compared with the native plasma. On-particle digestion was ultimately performed to release all proteins retained on ampholine@PM. Followed by MuPIT analysis, the number of identified proteins in plasma was improved by 75% after ampholine@PM treatment. Furthermore, the spectral count of 9 high abundance proteins was decreased by 37.6-97.2%, and the identified low abundance protein (< 100 ng mL(-1)) number was increased from 4 to 17. These results demonstrated that the fractionation by ampholine@PM could efficiently decrease the protein dynamic range in abundance, beneficial to achieve the deep coverage identification of human plasma proteome. (c) 2014 Elsevier B. V. All rights reserved.
语种英语
WOS记录号WOS:000335452300006
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/145916
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Deng, Nan,Zhu, Guijie,Chen, Yuanbo,et al. Decrease of dynamic range of proteins in human plasma by ampholine immobilized polymer microspheres[J]. ANALYTICA CHIMICA ACTA,2014,826:43-50.
APA Deng, Nan.,Zhu, Guijie.,Chen, Yuanbo.,Wu, Qi.,Liang, Zhen.,...&Zhang, Yukui.(2014).Decrease of dynamic range of proteins in human plasma by ampholine immobilized polymer microspheres.ANALYTICA CHIMICA ACTA,826,43-50.
MLA Deng, Nan,et al."Decrease of dynamic range of proteins in human plasma by ampholine immobilized polymer microspheres".ANALYTICA CHIMICA ACTA 826(2014):43-50.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Deng, Nan]的文章
[Zhu, Guijie]的文章
[Chen, Yuanbo]的文章
百度学术
百度学术中相似的文章
[Deng, Nan]的文章
[Zhu, Guijie]的文章
[Chen, Yuanbo]的文章
必应学术
必应学术中相似的文章
[Deng, Nan]的文章
[Zhu, Guijie]的文章
[Chen, Yuanbo]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。