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题名: High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl beta-D-Maltoside
作者: Liu, Jing1, 2;  Wang, Fangjun1;  Mao, Jiawei1, 2;  Zhang, Zhang1, 2;  Liu, Zheyi1, 2;  Huang, Guang1, 2;  Cheng, Kai1, 2;  Zou, Hanfa1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2015-02-17
DOI: 10.1021/ac504700t
卷: 87, 期:4, 页:2054-2057
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: N-dodecyl beta-D-maltoside (DDM), a mild detergent with the ability to maintain the enzyme activity and solubilize hydrophobic proteins without changing their structures, was applied for N-glycoproteomic analysis of minute protein sample from mouse brain tissue. After combining with the capillary-based glycoproteomic reactor, 281 N-glycosylation sites were successfully characterized from 50 mu g of mouse brain tissue, which was 110% higher at least than those obtained by conventional strategies.
关键词[WOS]: SPECTROMETRY-COMPATIBLE SURFACTANTS ;  SAMPLE PREPARATION METHOD ;  MASS-SPECTROMETRY ;  MEMBRANE-PROTEINS ;  CELLS ;  PROTEOMICS ;  GLYCOSYLATION ;  PURIFICATION
语种: 英语
WOS记录号: WOS:000349806200007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145932
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Liu, Jing,Wang, Fangjun,Mao, Jiawei,et al. High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl beta-D-Maltoside[J]. ANALYTICAL CHEMISTRY,2015,87(4):2054-2057.
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