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题名: Development of Urinary Pseudotargeted LC-MS-Based Metabolomics Method and Its Application in Hepatocellular Carcinoma Biomarker Discovery
作者: Shao, Yaping1;  Zhu, Bin2;  Zheng, Ruiyin3;  Zhao, Xinjie1;  Yin, Peiyuan1;  Lu, Xin1;  Jiao, Binghua3;  Xu, Guowang1;  Yao, Zhenzhen3
关键词: pseudotargeted method ;  LC-MS ;  metabolomics ;  urine ;  cirrhosis ;  hepatocellular ;  carcinoma
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2015-02-01
DOI: 10.1021/pr500973d
卷: 14, 期:2, 页:906-916
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Hepatocellular carcinoma (HCC) is one of the pestilent malignancies leading to cancer-related death. Discovering effective biomarkers for HCC diagnosis is an urgent demand. To identify potential metabolite biomarkers, we developed a urinary pseudotargeted method based on liquid chromatography-hybrid triple quadrupole linear ion trap mass spectrometry (LC-QTRAP MS). Compared with nontargeted method, the pseudotargeted method can achieve better data quality, which benefits differential metabolites discovery. The established method was applied to cirrhosis (CIR) and HCC investigation. It was found that urinary nucleosides, bile acids, citric acid, and several amino acids were significantly changed in liver disease groups compared with the controls, featuring the dysregulation of purine metabolism, energy metabolism, and amino metabolism in liver diseases. Furthermore, some metabolites such as cyclic adenosine monophosphate, glutamine, and short- and medium-chain acylcarnitines were the differential metabolites of HCC and CIR. On the basis of binary logistic regression, butyrylcarnitine (carnitine C4:0) and hydantoin-5-propionic acid were defined as combinational markers to distinguish HCC from CIR. The area under curve was 0.786 and 0.773 for discovery stage and validation stage samples, respectively. These data show that the established pseudotargeted method is a complementary one of targeted and nontargeted methods for metabolomics study.
关键词[WOS]: CHROMATOGRAPHY-MASS SPECTROMETRY ;  LIVER-CIRRHOSIS ;  SERUM METABOLOMICS ;  TUMOR-MARKERS ;  HEPATITIS-B ;  CANCER ;  METABOLISM ;  NUCLEOSIDES ;  DIAGNOSIS ;  ACYLCARNITINES
语种: 英语
WOS记录号: WOS:000349276400030
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/145947
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Dept Biliary Surg 2, Shanghai 200438, Peoples R China
3.Second Mil Med Univ, Dept Biochem & Mol Biol, Shanghai 200433, Peoples R China

Recommended Citation:
Shao, Yaping,Zhu, Bin,Zheng, Ruiyin,et al. Development of Urinary Pseudotargeted LC-MS-Based Metabolomics Method and Its Application in Hepatocellular Carcinoma Biomarker Discovery[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(2):906-916.
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