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题名: High-Performance Liquid Chromatography Separation of cis-trans Anthocyanin Isomers from Wild Lycium ruthenicum Murr. Employing a Mixed-Mode Reversed-Phase/Strong Anion-Exchange Stationary Phase
作者: Jin, Hongli1, 2, 3;  Liu, Yanfang2;  Guo, Zhimou2;  Yang, Fan4;  Wang, Jixia2, 3;  Li, Xiaolong1, 2, 3;  Peng, Xiaojun1;  Lang, Xinmiao2
关键词: anthocyanins ;  cis-trans anthocyanin isomer ;  Lycium ruthenicum Murr. ;  mixed-mode ;  stationary phase ;  HPLC
刊名: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
发表日期: 2015-01-21
DOI: 10.1021/jf504525w
卷: 63, 期:2, 页:500-508
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Agriculture, Multidisciplinary ;  Chemistry, Applied ;  Food Science & Technology
研究领域[WOS]: Agriculture ;  Chemistry ;  Food Science & Technology
英文摘要: The cis-trans isomerism is a common phenomenon for acylated anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure anthocyanins, including two pairs of cis-trans isomeric anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the results indicated that this method is an effective way to separate anthocyanins, especially for cis-trans isomers.
关键词[WOS]: RED WINE ANTHOCYANINS ;  MASS-SPECTROMETRY ;  IDENTIFICATION ;  GRAPES ;  COLUMN ;  FRUITS
语种: 英语
WOS记录号: WOS:000348332500017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146016
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Liaoning, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Analyt Chem Serv Ctr, Dalian 116023, Liaoning, Peoples R China

Recommended Citation:
Jin, Hongli,Liu, Yanfang,Guo, Zhimou,et al. High-Performance Liquid Chromatography Separation of cis-trans Anthocyanin Isomers from Wild Lycium ruthenicum Murr. Employing a Mixed-Mode Reversed-Phase/Strong Anion-Exchange Stationary Phase[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2015,63(2):500-508.
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