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High-Performance Liquid Chromatography Separation of cis-trans Anthocyanin Isomers from Wild Lycium ruthenicum Murr. Employing a Mixed-Mode Reversed-Phase/Strong Anion-Exchange Stationary Phase
Jin, Hongli1,2,3; Liu, Yanfang2; Guo, Zhimou2; Yang, Fan4; Wang, Jixia2,3; Li, Xiaolong1,2,3; Peng, Xiaojun1; Lang, Xinmiao2
关键词Anthocyanins Cis-trans Anthocyanin Isomer Lycium Ruthenicum Murr. Mixed-mode Stationary Phase Hplc
刊名JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
2015-01-21
DOI10.1021/jf504525w
63期:2页:500-508
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]Agriculture ; Chemistry ; Food Science & Technology
关键词[WOS]RED WINE ANTHOCYANINS ; MASS-SPECTROMETRY ; IDENTIFICATION ; GRAPES ; COLUMN ; FRUITS
英文摘要The cis-trans isomerism is a common phenomenon for acylated anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure anthocyanins, including two pairs of cis-trans isomeric anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the results indicated that this method is an effective way to separate anthocyanins, especially for cis-trans isomers.
语种英语
WOS记录号WOS:000348332500017
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被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146016
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Liaoning, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Analyt Chem Serv Ctr, Dalian 116023, Liaoning, Peoples R China
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Jin, Hongli,Liu, Yanfang,Guo, Zhimou,et al. High-Performance Liquid Chromatography Separation of cis-trans Anthocyanin Isomers from Wild Lycium ruthenicum Murr. Employing a Mixed-Mode Reversed-Phase/Strong Anion-Exchange Stationary Phase[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2015,63(2):500-508.
APA Jin, Hongli.,Liu, Yanfang.,Guo, Zhimou.,Yang, Fan.,Wang, Jixia.,...&Lang, Xinmiao.(2015).High-Performance Liquid Chromatography Separation of cis-trans Anthocyanin Isomers from Wild Lycium ruthenicum Murr. Employing a Mixed-Mode Reversed-Phase/Strong Anion-Exchange Stationary Phase.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,63(2),500-508.
MLA Jin, Hongli,et al."High-Performance Liquid Chromatography Separation of cis-trans Anthocyanin Isomers from Wild Lycium ruthenicum Murr. Employing a Mixed-Mode Reversed-Phase/Strong Anion-Exchange Stationary Phase".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63.2(2015):500-508.
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