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题名: Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism
作者: Liu, Yinghui1;  Feng, Yanbin1;  Wang, Yayue1, 2;  Li, Xia3;  Cao, Xupeng1;  Xue, Song1
关键词: MCAT ;  Synechocystis sp PCC 6803 ;  Structure ;  Intermediate
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2015-02-13
DOI: 10.1016/j.bbrc.2015.01.003
卷: 457, 期:3, 页:398-403
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics
英文摘要: Malonyl-coenzyme A: acyl-carrier protein transacylase (MCAT) catalyzes the transfer of malonyl group from malonyl-CoA to the holo-acyl carrier protein (Holo-ACP), yielding malonyl-ACR The overall reaction has been extensively studied in heterotrophic microorganisms, while its mechanism in photosynthetic autotrophs as well as the stepwise reaction information remains unclear. Here the 2.42 angstrom crystal structure of MCAT from photosynthetic microorganism Synechocystis sp. PCC 6803 is presented. It demonstrates that Arg113, Ser88 and His188 constitute catalytic triad. The second step involved ACPMCAT-malonyl intermediate is speed-limited instead of the malonyl-CoA-MCAT intermediate in the first step. Therefore His87, Arg113 and Ser88 render different contributions for the two intermediates. Additionally, S88T mutant initializes the reaction by H87 deprotonating S88T which is different from the wild type. (C) 2015 Elsevier Inc. All rights reserved.
关键词[WOS]: ACYL CARRIER PROTEIN ;  MALONYL-COA ;  CRYSTAL-STRUCTURE ;  ESCHERICHIA-COLI ;  STREPTOMYCES-COELICOLOR ;  HELICOBACTER-PYLORI ;  TRANSACYLASE ;  BIOSYNTHESIS ;  SPECIFICITY ;  RESOLUTION
语种: 英语
WOS记录号: WOS:000351254700027
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146087
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Dalian Polytech Univ, Dalian 116034, Peoples R China

Recommended Citation:
Liu, Yinghui,Feng, Yanbin,Wang, Yayue,et al. Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,457(3):398-403.
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