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Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism
Liu, Yinghui1; Feng, Yanbin1; Wang, Yayue1,2; Li, Xia3; Cao, Xupeng1; Xue, Song1
关键词Mcat Synechocystis Sp Pcc 6803 Structure Intermediate
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2015-02-13
DOI10.1016/j.bbrc.2015.01.003
457期:3页:398-403
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]ACYL CARRIER PROTEIN ; MALONYL-COA ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; STREPTOMYCES-COELICOLOR ; HELICOBACTER-PYLORI ; TRANSACYLASE ; BIOSYNTHESIS ; SPECIFICITY ; RESOLUTION
英文摘要Malonyl-coenzyme A: acyl-carrier protein transacylase (MCAT) catalyzes the transfer of malonyl group from malonyl-CoA to the holo-acyl carrier protein (Holo-ACP), yielding malonyl-ACR The overall reaction has been extensively studied in heterotrophic microorganisms, while its mechanism in photosynthetic autotrophs as well as the stepwise reaction information remains unclear. Here the 2.42 angstrom crystal structure of MCAT from photosynthetic microorganism Synechocystis sp. PCC 6803 is presented. It demonstrates that Arg113, Ser88 and His188 constitute catalytic triad. The second step involved ACPMCAT-malonyl intermediate is speed-limited instead of the malonyl-CoA-MCAT intermediate in the first step. Therefore His87, Arg113 and Ser88 render different contributions for the two intermediates. Additionally, S88T mutant initializes the reaction by H87 deprotonating S88T which is different from the wild type. (C) 2015 Elsevier Inc. All rights reserved.
语种英语
WOS记录号WOS:000351254700027
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146087
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Dalian Polytech Univ, Dalian 116034, Peoples R China
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Liu, Yinghui,Feng, Yanbin,Wang, Yayue,et al. Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,457(3):398-403.
APA Liu, Yinghui,Feng, Yanbin,Wang, Yayue,Li, Xia,Cao, Xupeng,&Xue, Song.(2015).Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,457(3),398-403.
MLA Liu, Yinghui,et al."Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 457.3(2015):398-403.
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