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Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism
Liu, Yinghui1; Feng, Yanbin1; Wang, Yayue1,2; Li, Xia3; Cao, Xupeng1; Xue, Song1
KeywordMcat Synechocystis Sp Pcc 6803 Structure Intermediate
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2015-02-13
DOI10.1016/j.bbrc.2015.01.003
Volume457Issue:3Pages:398-403
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS KeywordACYL CARRIER PROTEIN ; MALONYL-COA ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; STREPTOMYCES-COELICOLOR ; HELICOBACTER-PYLORI ; TRANSACYLASE ; BIOSYNTHESIS ; SPECIFICITY ; RESOLUTION
AbstractMalonyl-coenzyme A: acyl-carrier protein transacylase (MCAT) catalyzes the transfer of malonyl group from malonyl-CoA to the holo-acyl carrier protein (Holo-ACP), yielding malonyl-ACR The overall reaction has been extensively studied in heterotrophic microorganisms, while its mechanism in photosynthetic autotrophs as well as the stepwise reaction information remains unclear. Here the 2.42 angstrom crystal structure of MCAT from photosynthetic microorganism Synechocystis sp. PCC 6803 is presented. It demonstrates that Arg113, Ser88 and His188 constitute catalytic triad. The second step involved ACPMCAT-malonyl intermediate is speed-limited instead of the malonyl-CoA-MCAT intermediate in the first step. Therefore His87, Arg113 and Ser88 render different contributions for the two intermediates. Additionally, S88T mutant initializes the reaction by H87 deprotonating S88T which is different from the wild type. (C) 2015 Elsevier Inc. All rights reserved.
Language英语
WOS IDWOS:000351254700027
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146087
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Dalian Polytech Univ, Dalian 116034, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yinghui,Feng, Yanbin,Wang, Yayue,et al. Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,457(3):398-403.
APA Liu, Yinghui,Feng, Yanbin,Wang, Yayue,Li, Xia,Cao, Xupeng,&Xue, Song.(2015).Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,457(3),398-403.
MLA Liu, Yinghui,et al."Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 457.3(2015):398-403.
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